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Table 1 Oligonucleotide sequence for the amplification of mecA, orfX and SCCmec types

From: In vitro transfer of methicillin resistance determinants mecA from methicillin resistant Staphylococcus aureus (MRSA) to methicillin susceptible Staphylococcus aureus (MSSA)

S/N

Primer

Oligonucleotide sequence 5’to 3′

Product size

Annealing temperature

Reference

1

mecAF mecAR

ACTGCTATCCACCCTCAAAC

163 bp

57 °C/120 s

Noto. [27]; Mehrotra et al. [28]

CTGGTGAAGTTGTAATCTG

2

Nuc-F

GCG ATT GAT GG TGA TAC GGT T

276 bp

55 °C/120 s

Saiful et al. [29]

Nuc-R

AGC CAA CGG TTG ACG AAC TAA AGC

3

OrfX F

GAG AAA TAT TGG AAG CAA GCC

326 bp

54.6 °C/60s

Noto. [27]

OrfX R

CGC ATA ATC TTA AAT GCT CTG

4

SCCmecIIIFSCCmecIIIR

TTC TCA TTG ATG CTG AAG CC

280 bp

55 °C /60 s

Zhang et al. [30]

GTG TAA TTT CTT TTG AAA GAT ATG G

5

mecA F

5′-ACTGCTATCCACCCTCAAAC-3′

533 bp

55 °C/120 s

Saiful et al. [29]

mecA R

5′-CTGGTGAAGTTGTAATCTGG-3′