Fig. 5.

C. caviae and C. trachomatis are inversely sensitive to ampicillin and LPC. a C. caviae infected cells were treated with 0.25 μg/mL LPC at 0 hpi and at 5, 24, and 45 hpi cells were washed and drug was removed. At 48 hpi, infected cells were methanol fixed, and labeled with the anti-LOS mAb (green) and anti-Hsp60 mAb (red) and total DNA with DAPI (blue). At 24 hpi, a modest production of LOS was observed within C. caviae inclusions. b C. caviae and C. trachomatis L2-infected cells were treated with LPC at their respective MECs for 24 hpi, and allowed to recover for an additional 24 h in the absence of drug. Cells were then labeled and visualized as in (a). c Cells were treated with ampicillin in place of LPC. d and e Infected cells were treated with LPC (d) or ampicillin (e) and allowed to recover as above. Infectious progeny titers were determined by re-infecting McCoy cells (*, P < 0.05). Scale bar is 10 μm