Skip to main content

Table 2 Gene Expression Effects of Probiotic Bacteria on S. enterica-Challenged Mice (Fold Change in mRNA Expression vs. Control)

From: Probiotic bacteria prevent Salmonella – induced suppression of lymphoproliferation in mice by an immunomodulatory mechanism

Gene-coded protein/Function Fold Changea
B and T cell activation genesb
Cd2/Induces IFN-γ, interacts with PTPRC + 2.9
Cd21 (Cr2)/B cell complement receptor + 3.0
Hells/Helicase - Cell growth, DNA methylation + 2.5
Hsp90aa1/AKT signal transduction pathway, protein chaperon + 2.3
Il4/TH-2 cytokine (IL-4) - 2.6
Il10/TH-2 cytokine (IL-10) + 3.6
Il12b/TH-1 cytokine (IL-12p40) - 2.7
Impdh/Cell growth + 2.5
Nkx2.3/Lymphocyte cellular differentiation + 2.3
Prkcd/B-cell signal transduction and apoptosis + 2.2
Prlr/Prolactin receptor + 2.1
Ptprc/(CD45/B220) cell growth/differentiation + 3.0
Tlr1/Proinflammatory receptor for microbes - 2.5
Tlr6/Proinflammatory receptor for microbes + 2.3
Vav1/T and B cell activation via JNK and P38 + 2.1
Wwp1/E3 ubiquitin protein ligase + 2.4
Zap70/T cell receptor cofactor - 2.4
Nf-κb signal transduction genesc  
Bcl2/Anti-apoptotic signaling - 1.1
Bcl2l10/Pro-apoptotic, pro-inflammatory signaling - 1.8
Cflar/Pro-apoptotic signaling - 1.2
Egr1/B-cell receptor signaling - 2.1
Fasl/Intercellular signaling, pro-apoptotic - 3.3
Fos/Intracellular signaling, pro-apoptotic - 3.1
Icam1/Leukocyte movement, signaling - 2.3
Iκκa (Chuk)/Pro-inflammatory activator of NF-κB - 2.1
Irak2/Induces NFκB nuclear translocation + 2.3
Jun/(AP-1) B-cell and T-cell receptor signaling + 2.1
Mekk/(Map3k1) Pro-inflammatory - 2.2
Myd88/Pro-apoptotic and inflammatory adapter - 3.8
Nfkbia/Blocks NFκB nuclear translocation - 2.1
Relb/NFκB cofactor activation in B-cells - 3.0
Ripk1/Pro-inflammatory, apoptosis regulatory - 1.1
Rnf7/IκB ubiquitination – pro-apoptotic, pro-inflammatory - 3.8
Traf2/Pro-inflammatory, apoptosis regulatory - 1.7
Apoptosis genesd
Akt1/Anti-apoptotic - 2.1
Bad/Anti- apoptotic - 2.2
Card10/Proinflammatory via NFκB, pro-apoptotic - 4.3
Casp2/Pro-apoptotic - 2.3
Casp12/Pro-apoptotic - 2.8
Dad1/Possible apoptosis inhibitor - 3.1
Fadd/Pro-apoptotic - 2.2
Pak7/Protein kinase induces proliferation/ anti-apoptotic - 4.2
Tnf/Pro-apoptotic cytokine - 2.4
  1. aThe mean (n = 4/group) fold change in qRT-PCR threshold cycles (CT) between tissue RNA samples amplified from probiotic-treated versus untreated control mice were calculated as 2(−ΔΔCT), where ΔCT is the housekeeping gene-normalized average CT and ΔΔCT is the ΔCT(treatment)- ΔCT(control). Positive values indicate increased expression and negative values indicate reduced expression
  2. bResults from Qiagen PAMM-053 Mouse T and B Cell Activation Array
  3. cResults from Qiagen PAMM-025 Mouse NF-κB Array
  4. dResults from Qiagen PAMM-012 Mouse Apoptosis Array