Table 4 Amino acids content of purified cholesterol oxidase produced by Streptomyces aegyptia NEAE 102
Amino acid | Residue concentration | Estimated no. of residues/mola (to nearest integer) | |||
|---|---|---|---|---|---|
μg/ml | nmol/ml | g/mol | Mol.% |  | |
Hydrophobic | |||||
Aliphatic | |||||
 Glycine (Gly) | 5.53 | 73.71 | 75.06 | 10.76 | 39 |
 Alanine (Ala) | 7.77 | 87.26 | 89.09 | 12.73 | 46 |
 Isoleucine (Ile) | 3.46 | 26.40 | 131.17 | 3.85 | 14 |
 Leucine (Leu) | 10.28 | 78.71 | 130.67 | 11.49 | 42 |
 Valine (Val) | 3.66 | 31.21 | 117.14 | 4.55 | 16 |
Aromatic | |||||
 Phenylalanine (Phe) | 5.71 | 34.54 | 165.17 | 5.04 | 18 |
Charged | |||||
Acidic | |||||
 Aspartic acid (Asp + Asn) | 5.62 | 42.24 | 133.00 | 6.16 | 22 |
 Glutamic acid (Glu + Gln) | 11.49 | 78.12 | 147.11 | 11.40 | 41 |
Basic | |||||
 Histidine (His) | 5.91 | 38.10 | 155.17 | 5.56 | 20 |
 Lysine (Lys) | 5.48 | 37.46 | 146.18 | 5.47 | 20 |
 Arginine (Arg) | 8.67 | 49.79 | 174.19 | 7.27 | 26 |
Polar | |||||
 Serine (Ser) | 4.31 | 40.96 | 105.10 | 5.98 | 22 |
 Threonine (Thr) | 5.27 | 44.26 | 119.11 | 6.46 | 23 |
 Methionine (Met) | 0.33 | 2.21 | 148.42 | 0.32 | 1 |
Aromatic | |||||
 Tyrosine (Tyr) | 3.67 | 20.28 | 181.16 | 2.96 | 11 |
 Total |  |  |  |  | 361 |