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Table 4 Amino acids content of purified cholesterol oxidase produced by Streptomyces aegyptia NEAE 102

From: Purification, characterization and amino acid content of cholesterol oxidase produced by Streptomyces aegyptia NEAE 102

Amino acid Residue concentration Estimated no. of residues/mola
(to nearest integer)
μg/ml nmol/ml g/mol Mol.%  
Hydrophobic
Aliphatic
 Glycine (Gly) 5.53 73.71 75.06 10.76 39
 Alanine (Ala) 7.77 87.26 89.09 12.73 46
 Isoleucine (Ile) 3.46 26.40 131.17 3.85 14
 Leucine (Leu) 10.28 78.71 130.67 11.49 42
 Valine (Val) 3.66 31.21 117.14 4.55 16
Aromatic
 Phenylalanine (Phe) 5.71 34.54 165.17 5.04 18
Charged
Acidic
 Aspartic acid (Asp + Asn) 5.62 42.24 133.00 6.16 22
 Glutamic acid (Glu + Gln) 11.49 78.12 147.11 11.40 41
Basic
 Histidine (His) 5.91 38.10 155.17 5.56 20
 Lysine (Lys) 5.48 37.46 146.18 5.47 20
 Arginine (Arg) 8.67 49.79 174.19 7.27 26
Polar
 Serine (Ser) 4.31 40.96 105.10 5.98 22
 Threonine (Thr) 5.27 44.26 119.11 6.46 23
 Methionine (Met) 0.33 2.21 148.42 0.32 1
Aromatic
 Tyrosine (Tyr) 3.67 20.28 181.16 2.96 11
 Total      361
  1. aThe number of calculated amino acid based on: if the MW of the purified enzyme is 46000 Da and average MW of the 20 most common aa is 110 Da, the no. of aa will be approximately 46000/110 = 418aa. Trp-residues cannot be determined, based on the methodology used. Pro residues were also not detectable. Because of the deletion of 2 of the 20 amino acids in the calculation: W, P (=10%), we have taken away 10% of aa