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Table 2 Half life time (T1/2) and heat deactivation constant (k) of cholesterol oxidase produced by Streptomyces aegyptia NEAE 102

From: Purification, characterization and amino acid content of cholesterol oxidase produced by Streptomyces aegyptia NEAE 102

Half life time (min)

 50 °C

156.25

 60 °C

107.27

 70 °C

81.82

 80 °C

80.33

Thermal inactivation rate constant k d 1 (min−1)a

 50 °C

0.0044

 60 °C

0.0065

 70 °C

0.0085

 80 °C

0.0086

D-value (min)

 50 °C

281.25

 60 °C

193.09

 70 °C

147.28

 80 °C

144.60

Thermal inactivation rate constant k d 2 (min−1)b

 50 °C

0.0025

 60 °C

0.0036

 70 °C

0.0047

 80 °C

0.0048

  1. a k d 1 is the deactivation constant after losing 50% of initial activity (at T1/2)
  2. b k d 2 is the deactivation constant after losing 90% of initial activity (at D- value)