Table 1 Stool temperature storage conditions

1

Immediately frozen -80 °C

2

Held overnight at 4 °C, then frozen -80 °C

3

48 h at 4 °C, then frozen -80 °C

4

Immediately frozen -20 °C for 24 h, 1 thaw cycle, frozen at -80 °C

5

Immediately frozen -20 °C for 24 h, 1^st thaw, frozen -20 °C, 2^nd thaw, frozen at -80 °C