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Table 2 Efficiency of P1 transduction: complementation by different pnp mutant alleles

From: Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli

Plasmida pnp allele on plasmid trpE leuA
TFb (×107) TF fractionc TFb (×107) TF fractionc
pAZ101 wt 2.53 (±0.09) 1.00 4.40 (±0.35) 1.00
pGZ119HE none 0.75 (±0.20) 0.30 1.34 (±0.30) 0.30
pAZ1112 S438A 2.07 (±0.52) 0.82 2.70 (±0.46) 0.60
pAZ1113 ΔKH 0.55 (±0.15) 0.22 1.18 (±0.24) 0.26
pAZ1114 ΔS1 0.53 (±0.19) 0.21 1.19 (±0.25) 0.27
pAZ133 ΔKH-S1 0.06 (±0.02) 0.02 0.17 (±0.06) 0.04
  1. ain C-5691 (Δpnp) strain
  2. btransduction frequency (TF) is the ratio of transductants to adsorbed phage
  3. cratio to TF of wild type strain