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Table 1 P1 transduction in Δpnp strains

From: Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli

Transduced marker

trpE

leuA

Chromosomal pnp allele

Adsorbed phagea

m.o.i.b

TFc (Ă—107)

TF fractiond

Adsorbed phagea

m.o.i.b

TFc (Ă—107)

TF fractiond

wild type

0.98 (±0.01)

5.87Ă—10-2

37.0 (±1.4)

1.00

0.98 (±0.01)

5.66Ă—10-2

23.0 (±1.2)

1.00

Δpnp-751

0.97 (±0.01)

5.84Ă—10-2

6.9 (±0.28)

0.19

0.97 (±0.01)

5.81Ă—10-2

3.7 (±0.17)

0.16

  1. aadsorbed phage is input phage - unadsorbed phage as assayed 20 min post infection
  2. bmultiplicity of infection (m.o.i.) is the ratio of phage to bacterial cells
  3. ctransduction frequency (TF) is the ratio of transductants to adsorbed phage. Average and standard deviation of three experiments are reported
  4. dratio to TF of wild type strain