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Fig. 4 | BMC Microbiology

Fig. 4

From: Polynucleotide phosphorylase is implicated in homologous recombination and DNA repair in Escherichia coli

Fig. 4

Electrophoretic mobility shift assay of PNPase with RNA and DNA oligonucleotides. [32P]-5'-end labeled RNA (RNA01; panel a) or ssDNA (DNA02; panel b) 20-mers were incubated 20 min at 21 °C with wild type or ΔKHS1 mutant His-tagged PNPase at the concentrations indicated on the top of the panels. 2 mM MnCl2 or MgCl2 were added to Binding Buffer as specified. PNPase-nucleic acid complexes were resolved by native 5% PAGE. After electrophoresis the gels were dried and the autoradiographic images analyzed by phosphorimaging and ImageQuant software

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BMC Microbiology

ISSN: 1471-2180

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