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Table 4 Detection of virulence genes by multiplex PCRa

From: Uropathogenic Escherichia coli pathogenicity islands and other ExPEC virulence genes may contribute to the genome variability of enteroinvasive E. coli

  Gene (amplicon bp) Annealing condition Reference for multiplex composition
Multiplex 1 afaBCIII (750)
papC (328)
sfaDE (410)
65 °C – 1 min [47]
Multiplex 2 cvaC (680)
KpsMT II (272)
ompT (559)
58 °C – 30 s This study
Multiplex 3 hlyA (1,177)
iroN (665)
papA (720)
traT (290)
58 °C – 30 s This study
Multiplex 4 ompA (919)
hra (537)
58 °C – 30 s This study
Multiplex 5 iha (925)
sat (667)
sitA (608)
58 °C – 30 sec This study
  1. aprimers for multiplex PCR reactions 2, 3, 4, and 5 were described elsewhere [46]. Genes chuA, iucD,irp2, vat, and fimA were searched by single PCR reactions [46]