Fig. 4

Mutational Analysis of Hfq3. a, b Jmol-generated images of the proximal and distal faces of E. coli Hfq (PDB 3QHS) with mutated residues highlighted and numbered using B. anthracis Hfq3 nomenclature. c Growth comparison of each Hfq3 mutant patched to LB incubated at 37 °C in air (left) or to NBY/bicarbonate incubated at 37 °C in 15% CO₂ (right). Mutations are noted as “P” for proximal, “D” for distal, or “R” for rim along the edges of the plate. d Expression of each Hfq3 mutant grown under inducing conditions to an OD₆₀₀ of 0.7–1.0. Note that the Hfq3 control was prepared as described in Fig. 2d. Equal amounts of total soluble protein from cell lysates were subjected to SDS-PAGE and western blotting with anti-Hfq3. Samples were analyzed under semi-native (-) and boiled (+) conditions. Dotted arrows represent the 50 kDa marker band