Clonality, outer-membrane proteins profile and efflux pump in KPC- producing Enterobacter sp. in Brazil

BMC Microbiology

Table 5 Outer membrane proteins, blaKPC gene, blaTEM and blaCTX and pump efflux (AcrART) profile of 22 carbapenems-resistant isolates of E. aerogenes (hospital A and B) and 5 E. cloacae (hospital C)

			Imipenem	Meropenem	Ertapenem	PCR				SDS-PAGE
PFGE	Hospital	Date	MIC	MIC	MIC	bla KPC	bla CTX	bla TEM 1	acrART	42 KDa	39 Kda	35 e 36 KDa
A2	A	13/05/2005	32	8	32	+	+	+	-	++++	+++	++
A2	A	10/12/2005	16	8	64	+	+	+	-	++++	+++	++
C11	A	18/05/2006	32	16	128	+	+	+	-	++++	+++	++
C11	A	13/07/2006	32	16	128	+	+	+	-	++++	++++	++
C8	A	14/07/2006	16	16	64	+	+	+	-	++++	++++	++
C7	A	02/01/2007	16	4	32	+	+	+	-	++++	Absent	++
C6	A	23/03/2007	16	8	64	+	+	+	-	++++	+++	++
C13	A	25/04/2007	8	4	32	+	+	+	-	+++	Absent	++
C14	A	21/09/2007	8	4	64	+	+	+	-	++++	+++	++
G1	A	10/11/2008	32	16	64	+	-	-	-	+++	Absent	++
C1	A	06/01/2009	16	32	64	+	-	+	-	+++	Absent	++
C2	A	31/08/2009	16	4	16	+	+	+	-	+++	++	++
C4	A	03/11/2009	32	16	64	+	+	+	-	+++	Absent	++
C5	A	29/11/2009	16	4	32	+	+	+	-	+++	+	++
C3	A	05/12/2009	4	4	16	+	+	+	-	+++	++	++
B1	A	16/03/2010	16	8	64	+	+	+	-	+++	Absent	++
F1	A	08/06/2011	128	64	≥128	+	-	+	-	+++	++	Absent
D2	B	26/12/2010	2	8	32	+	+	+	-	++++	+	++
D1	B	05/01/2011	4	8	32	+	+	+	-	++++	+	++
D1	B	05/01/2011	4	8	32	+	+	+	-	++++	+	++
D3	B	06/01/2011	4	8	8	+	+	+	-	+++	+	++
A2	C	19/11/2012	8	2	8	+	-	+	+	+++	+	Absent
B1	C	-	8	8	64	+	-	+	+	+++	+	Absent
B1	C	-	8	8	64	+	-	+	+	+++	+	Absent
A3	C	-	64	16	64	+	-	+	+	+++	+	Absent
A5	C	-	16	16	64	+	-	+	+	+++	+	Absent

ISSN: 1471-2180