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Table 5 Outer membrane proteins, blaKPC gene, blaTEM and blaCTX and pump efflux (AcrART) profile of 22 carbapenems-resistant isolates of E. aerogenes (hospital A and B) and 5 E. cloacae (hospital C)

From: Clonality, outer-membrane proteins profile and efflux pump in KPC- producing Enterobacter sp. in Brazil

  Imipenem Meropenem Ertapenem PCR SDS-PAGE
PFGE Hospital Date MIC MIC MIC bla KPC bla CTX bla TEM 1 acrART 42 KDa 39 Kda 35 e 36 KDa
A2 A 13/05/2005 32 8 32 + + + - ++++ +++ ++
A2 A 10/12/2005 16 8 64 + + + - ++++ +++ ++
C11 A 18/05/2006 32 16 128 + + + - ++++ +++ ++
C11 A 13/07/2006 32 16 128 + + + - ++++ ++++ ++
C8 A 14/07/2006 16 16 64 + + + - ++++ ++++ ++
C7 A 02/01/2007 16 4 32 + + + - ++++ Absent ++
C6 A 23/03/2007 16 8 64 + + + - ++++ +++ ++
C13 A 25/04/2007 8 4 32 + + + - +++ Absent ++
C14 A 21/09/2007 8 4 64 + + + - ++++ +++ ++
G1 A 10/11/2008 32 16 64 + - - - +++ Absent ++
C1 A 06/01/2009 16 32 64 + - + - +++ Absent ++
C2 A 31/08/2009 16 4 16 + + + - +++ ++ ++
C4 A 03/11/2009 32 16 64 + + + - +++ Absent ++
C5 A 29/11/2009 16 4 32 + + + - +++ + ++
C3 A 05/12/2009 4 4 16 + + + - +++ ++ ++
B1 A 16/03/2010 16 8 64 + + + - +++ Absent ++
F1 A 08/06/2011 128 64 ≥128 + - + - +++ ++ Absent
D2 B 26/12/2010 2 8 32 + + + - ++++ + ++
D1 B 05/01/2011 4 8 32 + + + - ++++ + ++
D1 B 05/01/2011 4 8 32 + + + - ++++ + ++
D3 B 06/01/2011 4 8 8 + + + - +++ + ++
A2 C 19/11/2012 8 2 8 + - + + +++ + Absent
B1 C - 8 8 64 + - + + +++ + Absent
B1 C - 8 8 64 + - + + +++ + Absent
A3 C - 64 16 64 + - + + +++ + Absent
A5 C - 16 16 64 + - + + +++ + Absent