Clonality, outer-membrane proteins profile and efflux pump in KPC- producing Enterobacter sp. in Brazil

BMC Microbiology

Table 1 Primers of all carbapenems resistance genes studied and PCR annealing temperature

Primers	Sequences (5′-3′)	Annealing temperature	Size (pb)
blaTEM - F	TCGCCGCATACACTATTCTCAGAATGA	55	420
blaTEM - R	ACG CTC ACC GGC TCC AGA TTT AT	55	420
blaCTXM - F	GCT CTAGAATTATTGCATCAGAAA CCGTG	55	893
blaCTXM - R	CGGAATTCATGATGACTCAGAGCATTGG	55	893
bla _OSHV - F	TGCTTTGTTAATTCGGGCCAA	55	730
bla _SHV - R	ATGCGTTATATTCGCCTGTG	55	730
bla _OXA48 - F	GTAACAATGCTTGGTTCG	55	177
bla _OXA48 - R	TGTTTTTGGTGGCATCGA	55	177
blakpc - F	GTTACGCCAAAGGACGAAC		893
blakpc - R	TTTTCAGAGCCTTACTGCCC		893
bla _SPM -F	CCTTTTCCGCGACCTTGATC	59	798
bla _SPM - R	ATGCGCTTCATTCACGCAC	59	798
bla _SIM - F	GTACAAGGGATTCGGCATCG	58	569
bla _SIM - R	GTACAAGGGATTCGGCATCG	58	569
bla _IMP -F	TTGGAAAATTATATAATCCC	47	188
bla _IMP - R	CCAAACCACTAGGTTATC	47	188
bla _VIM - F	TTTGGTCGCATATCGCAAAG	60	382
bla _VIM- R	CCATTCAGCCCAGATCGGCAT	60	382
bla _NDM - F	GGCGGAATGGCTCATCACGA	60	375
bla _NDM - R	CGCAACACAGCCTGACTTTC	60	375
AcrART - F	GAT TAT GAT TCT GCC TTG GCCG	60	130
AcrART - R	CAA TGC GAC CGC TGA TAG GGG	60	130

ISSN: 1471-2180