Skip to main content

Advertisement

Table 2 Proteins of Fusobacterium nucleatum enhanced when incubated in cysteine-rich broth prior to protein extraction*

From: The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

Spot no. Protein Protein function
1 Glyceraldehyde-3-phosphate dehydrogenasea, b Glycolytic protein
2 Bifunctional penicillin tolerance protein LytB/ribosomal protein S1 RpsAa Translation
3, 4 Pyruvate kinaseb, c Glycolytic protein
5 Recombination protein Ad /Histidyl-tRNA synthetasee, f DNA repair/histidyl-tRNA aminoacylation
6, 8 Acetate kinaseb, g Acetyl-CoA biosynthetic process
7 Electron transfer flavoprotein subunit alphab Electron carrier activity
9 Phosphoglycerate kinaseb, h Glycolytic protein
10 Zn-dependent alcohol dehydrogenase and related dehydrogenaseb Oxidoreductase, zinc ion binding
11 Pyridoxal biosynthesis lyase PdxSb Pyridoxal 5′-phosphate
12 Butyrate-acetoacetate CoA-transferase subunit Bb Butyrate metabolism
13 Acetoacetate: butyrate/acetate coenzyme A transferaseb Butyrate metabolism
14 Iron-sulfur cluster-binding proteinb Iron and sulphur binding
15 (S)-2-hydroxy-acid oxidase chain Di /glycolate oxidase, subunit GlcDg Oxidoreductase
16, 17 Rubrerythrina Oxidoreductase, iron ion binding
18 PTS-system, N-acetylglucosamine-specific IIA componentb Phosphotransferase system
19 Mannose-1-phosphate guanylyl transferase (GDP)b GDP-mannose biosynthetic process, lipopolysaccharide biosynthetic process
20 Translation initiation inhibitorb Deaminase activity
21 Anti-sigma F factor antagonistb Regulation of transcription
  1. *Fusobacterium nucleatum OMGS 3938 was incubated in Todd Hewitt broth with and without cysteine. The spots that were enhanced when incubated in cysteine-rich broth were extracted for identification with LC- MS/MS
  2. a Fusobacterium nucleatum subsp. polymorphum ATCC 10953
  3. b Fusobacterium nucleatum subsp. nucleatum ATCC 25586
  4. c Fusobacterium sp. 7_1
  5. d Fusobacterium nucleatum subsp. nucleatum ATCC 23726
  6. e Fusobacterium sp. 4_1_13
  7. f Fusobacterium nucleatum ChDC F128
  8. g Fusobacterium periodonticum ATCC 33693
  9. h Desulfosporosinus sp. OT
  10. i Fusobacterium nucelatum subsp. vicentii ATCC 49256