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Table 1 Bacteria examined for hydrogen sulfide (H2S)-producing enzymes, identified with in-gel cysteine digestion and bismuth staininga

From: The proteins of Fusobacterium spp. involved in hydrogen sulfide production from L-cysteine

Species Subspecies Strain Broth
Fusobacterium canifelinum   CCUGb 66382 Todd Hewitt
Fusobacterium necrophorum funduliforme ATCCc 51357 Todd Hewitt
Fusobacterium necrophorum   CCUG 48192 Todd Hewitt
Fusobacterium nucleatum polymorphum ATCC 10953 Todd Hewitt
Fusobacterium nucleatum   OMGSd 3938e Todd Hewitt
Fusobacterium periodonticum   ATCC 33693 Todd Hewitt
Fusobacterium periodonticum   CCUG 66383 Todd Hewitt
Parvimonas micra   ATCC 33270 BHIf + 10% serum
Porphyromonas endodontalis   OMGS 1205 BHI + 10% serum
Porphyromonas gingivalis (W83)   OMGS 197 BHI
Porphyromonas gingivalis (381 F)   CCUG 14449 BHI
Prevotella intermedia   ATCC 25611 BHI
Prevotella tannerae   ATCC 51259 BHI
Treponema denticola   OMGS 3271g Spirochete brothh
  1. aBacterial species were grown in broth until OD600 of approximately 0.8. After washing and centrifugation, the cells were lysed and the proteins were separated in gel by molecular weight, before staining in bismuth(III)chloride solution containing cysteine. The cysteine-degrading proteins that produced H2S were identified in the assay by color change; Sulfide from H2S reacted with bismuth and formed bismuth sulfide, a black precipitate. Another set of gels were also stained with conventional Coomassie staining. All experiments were repeated at least once
  2. bCulture Collection University of Gothenburg
  3. cAmerican Type Culture Collection
  4. dOral Microbiology Gothenburg Sweden
  5. eOriginally received from Malmö (Badersten 5U)
  6. fBrain Heart Infusion broth with 2 mL/L menadione and 10 mL/L hemin
  7. gOriginally received from Dr R. Ellen, University of Toronto, Toronto, Canada
  8. hDawson JR, Ellen RP. Tip-oriented adherence to Treponema denticola to fibronectin. Infect Immun. 1990//;58(12):3924–8