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Fig. 4 | BMC Microbiology

Fig. 4

From: Disruption of rcsB by a duplicated sequence in a curli-producing Escherichia coli O157:H7 results in differential gene expression in relation to biofilm formation, stress responses and metabolism

Fig. 4

Validation of the transcriptomic data by phenotypic assays. Bacterial cultures were grown overnight in LB-No Salt broth at 28 °C on a shaker (170 rpm). For motility assays, 2 μl of undiluted cultures were spotted on the surface of a soft motility agar plate (a). For other assays, overnight cultures were diluted appropriately into a suitable medium, and incubated at desired temperatures as described under Methods. Aliquots (100 μl) were withdrawn at 0, 2, 4 and 6 h intervals for measuring acid resistance (b) or at 0, 15, 30 and 60 min intervals for measuring tolerance to oxidative (c), heat (d) and osmotic (e) stressors. Each time point represents means of three independent assays and error bars represent standard deviation of 2. The time point with an * above them indicates p < 0.05 when the mutant was compared to the parental strain

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BMC Microbiology

ISSN: 1471-2180

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