Table 2 Homologous recombination efficiency of individual transformations, as assessed by phenotypic spore color screening using A. oryzae pyrG and A. nidulans hisB as selection markers to delete the olvA gene in kusA - recipient strains MF42.2 and MA169.4
From: HisB as novel selection marker for gene targeting approaches in Aspergillus niger
Recipient strain | Transformed plasmid | No. of transformants | No. of positive mutants | Homologous integration efficiency [%] |
|---|---|---|---|---|
MF42.2 (hisB - , pyrG +) | pSE1.6 (AnidhisB) | 34 | 30 | 91.2 |
MA169.4 (pyrG -) | pAW34 (AopyrG) | 31 | 27 | 89 ± 1.9 |
90 | 81 | |||
35 | 30 | |||
21 | 19 |