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Table 1 Bacteria, plasmids and primers used in this study

From: Signature-tagged mutagenesis screening revealed a novel smooth-to-rough transition determinant of Salmonella enterica serovar Enteritidis

Material Description/purpose Source or reference
Strains
 SE C50041 Salmonella enterica serovar Enteritidis; Wild-type; smooth Hu et al., 2013 [15]
 SE C50041ΔspiC ΔspiC mutant of SE C50041; Recipient strain; smooth Zeng et al., 2015 [16]
E. coli. χ7213-pir Donor strain Gift from R. Curtiss III
E. coli. DH5α For cloning Purchased from Takara company
 SE C50041ΔspiC - rfbG::Tn5Km2(Cm) rfbG transposon mutant of SE C50041ΔspiC This study
 SE C50041ΔrfbG ΔrfbG mutant of SE C50041 by λ-Red recombination system This study
S. Pullorum S06004 Negative control in the motility assay Geng et al., 2009 [30]
Plasmids
 pUT mini-Tn5Km2(Cm) For cloning Constructed and stored in our laboratory
 pKD3 Cm cassette template Datsenko & Wanner, 2000 [17]
 pKD46 λ-Red recombinase expression Datsenko & Wanner, 2000 [17]
 pCP20 FLP recombinase expression Datsenko & Wanner, 2000 [17]
Primers
 Y linker 5’- CTGCTCGAATTCAAGCTTCT -3’ This study
 P6U 5’- GAGCTCGAATTCGGCCTAG -3’ This study
rfbG forward 5’- AGGGCTGTGGGAAAAAGGTAAAGCTCCGTGGAAAACCTGGGAGTAAGTAGTGTGTAGGCTGGAGCTGCTTC -3’ This study
rfbG reverse 5’- CTCACGCAGGTTATTTGCTGTCATTACT
TTGATTCCTTAAACTTATTTTCCATATGAATATCCTCCTTAG -3’
This study