Table 1 Bacteria, plasmids and primers used in this study
Material | Description/purpose | Source or reference |
|---|---|---|
Strains | ||
 SE C50041 | Salmonella enterica serovar Enteritidis; Wild-type; smooth | Hu et al., 2013 [15] |
 SE C50041ΔspiC | ΔspiC mutant of SE C50041; Recipient strain; smooth | Zeng et al., 2015 [16] |
  E. coli. χ7213-pir | Donor strain | Gift from R. Curtiss III |
  E. coli. DH5α | For cloning | Purchased from Takara company |
 SE C50041ΔspiC - rfbG::Tn5Km2(Cm) | rfbG transposon mutant of SE C50041ΔspiC | This study |
 SE C50041ΔrfbG | ΔrfbG mutant of SE C50041 by λ-Red recombination system | This study |
  S. Pullorum S06004 | Negative control in the motility assay | Geng et al., 2009 [30] |
Plasmids | ||
 pUT mini-Tn5Km2(Cm) | For cloning | Constructed and stored in our laboratory |
 pKD3 | Cm cassette template | Datsenko & Wanner, 2000 [17] |
 pKD46 | λ-Red recombinase expression | Datsenko & Wanner, 2000 [17] |
 pCP20 | FLP recombinase expression | Datsenko & Wanner, 2000 [17] |
Primers | ||
 Y linker | 5’- CTGCTCGAATTCAAGCTTCT -3’ | This study |
 P6U | 5’- GAGCTCGAATTCGGCCTAG -3’ | This study |
  rfbG forward | 5’- AGGGCTGTGGGAAAAAGGTAAAGCTCCGTGGAAAACCTGGGAGTAAGTAGTGTGTAGGCTGGAGCTGCTTC -3’ | This study |
  rfbG reverse | 5’- CTCACGCAGGTTATTTGCTGTCATTACT TTGATTCCTTAAACTTATTTTCCATATGAATATCCTCCTTAG -3’ | This study |