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Fig. 1 | BMC Microbiology

Fig. 1

From: Signature-tagged mutagenesis screening revealed a novel smooth-to-rough transition determinant of Salmonella enterica serovar Enteritidis

Fig. 1

Rough strain characteristics of ΔrfbG mutants. a SDS-PAGE with silver staining of LPS of ΔrfbG mutants (SE C50041ΔrfbG and SE C50041ΔspiC - rfbG::Tn5Km2(Cm)) compared to SE C50041 or SE C50041ΔspiC. b Agglutination was examined with O9 MAb, O9 factor rabbit antiserum and acriflavine. Pictures were taken within 5 min. c Visual aggregation and “percent aggregation” of ΔrfbG mutants (SE C50041ΔrfbG and SE C50041ΔspiC - rfbG::Tn5Km2(Cm)), SE C50041 and SE C50041ΔspiC cultures grown statically for 16 h at 37 °C. 1, SE C50041; 2, SE C50041ΔrfbG; 3, SE C50041ΔspiC; 4, SE C50041ΔspiC - rfbG::Tn5Km2(Cm)

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