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Table 1 Enterococcus cecorum isolates included in this study, their phenotypic and genotypic properties, chicken embryo lethality and serotype

From: Comparison of pathogenic and non-pathogenic Enterococcus cecorum strains from different animal species

PCR results for virulence genes
Isolate number Animal species/production type Classification of isolateb Colony morpholgy D-mannitol metabolism Cytolysin (cylA) Enterococcal surface protein (esp) Aggregation substance (asa1) Hyaluronidase (hyl) Gelatinase (gelE) Cell wall adhesin of E. faecium (efaAfm) Cell wall adhesin of E. faecalis (efaAfs) Sex pheromone (ccf) Chicken embryo lethality in %e Serotypef
x288/5-95 Broiler Commensal SCVc - - - + - - - - - 20.0 n.t.g
09/309/1/A Broiler Pathogenic Nd - - - - - - - - - 0.0 n.t.
09/310/1/A Broiler Pathogenic N - - + - + + - - - 60.0 n.t.
12/673/4/A Broiler Pathogenic N - - - - - - - - - 6.7 n.t.
13/655/2/B Broiler Commensal N - - + + - + - + - 100.0 n.t.
13/655/3/B Broiler Commensal N - - - - - + - - - 25.0 n.t.
14/086/4/A Broiler Pathogenic N - - - - - - - - - 86.7 n.t.
14/086/9/A Broiler Pathogenic N - - - - - - - - - 53.3 n.t.
14/093/1/A Broiler Pathogenic N - - - - - - - - - 35.7 n.t.
14/166/2/A Broiler Pathogenic N - - - - - - - - - 80.0 2
14/359/1/A Broiler Pathogenic N - - - - - - - - - 33.3 n.t.
15/218/1/A Broiler Pathogenic N - - - - - + - - - 6.7 2
15/827/1/A Broiler Pathogenic N - - - - - - - - - 80.0 2
15/828/1/A Broiler Pathogenic N - - - - - - - - - 46.2 1
15/839/1/A Broiler Pathogenic N - - - - - - - - - 46.7 2
x829/5c-01 Pekin duck Commensal SCV - - - - - - - - - 0.0 2
x1610/1-01 Pekin duck Commensal SCV - - - - - - - - - 0.0 n.t.
10/063/20/A Pekin duck Pathogenic N - - + - + - - - - 53.3 2
13/698/3/B Pekin duck Commensal N - - + - - - - - - 8.3 2
14/456/2/A Pekin duck Commensal N - - - - - - - - - 13.3 n.t.
D07-797-90/61 Pekin duck Pathogenic N - - + - + + - - - 60.0 1
D09-367-6 Pekin duck Commensal N - - - - - - - - - 0.0 n.t.
D10-455-1 Pekin duck Pathogenic N - - - - - - - - - 46.6 2
D11-0675-2-8-3 Pekin duck Pathogenic N - - - - - - - - - 0.0 n.t.
D12-0364-1-1-1 Pekin duck Pathogenic N - - - - - - - - - 64.3 n.t.
D14-2291-5-7-1 Pekin duck Pathogenic N - - - - - - - - - 0.0 2
x204/2b-95 Laying hen Commensal N - - - - - - - - - 20.0 n.t.
x242/3-96 Laying hen Commensal SCV - - - + - - - - - 0.0 2
12/284/4/A Laying hen Commensal N - - + - - + - - - 6.7 n.t.
13/428/1/A Laying hen Commensal N + - - - - - - - - 28.6 n.t.
13/429/1/A Laying hen Commensal N + - - - - - - - - 46.6 n.t.
15/308/6/D Laying hen Commensal N - - - - - - - - - 0.0 n.t.
x150/2-95 Turkey Commensal N - - - - - - - - - 0.0 2
D11-0325-3-1-2 Turkey Pathogenic N - - - - - - - - - 20.0 1
D11-1088-2-1-1 Turkey Commensal N - - - - - - - - - 33.3 n.t.
D12-0108-3-3-3 Turkey Commensal N - - - - - - - - - 13.3 n.t.
D13-0112-3-1-2 Turkey Commensal N - - - - - - - - - 13.3 1
x459/1-95 Pigeon Commensal N + - - + - - - - + 0.0 1
14/022/1/A Pigeon Commensal N - - - - - - - - - 0.0 1
D12-0869-2-1-1 Pigeon Commensal N + - - - - - - - - 0.0 1
D14-1051-5-1-4 Pigeon Commensal N - - - - - - - - - 6.7 n.t.
D12-0239-4-1-2 Cattle Commensal N + - - - - - - - - 66.7 1
D12-1662-1-14-1 Cattle Commensal N + - - - - - - - - 20.0 n.t.
D11-0174-10-1-3 Swine Commensal N + - - - - - - - - 93.3 n.t.
D13-0364-1-1-4 Swine Commensal N + - - - - + - - - 13.3 2
x23/1-95 Budgerigar Commensal N + - - - - - - - + 13.3 2
12/437/1/C Goose Commensal N - - - - - + - - + 6.7 n.t.
14/1103/1/A Human Pathogenic N - - - - - - - - - 13.3 n.t.
10/704/4/A Muscovy duck Pathogenic N - - - - - - - + + 40.0 n.t.
x495/3-95 Swan Commensal SCV - - - - - - - - - 0.0 1
DSM 20682 a Chicken Commensal N - - + - + - - - - 63.6 n.t.
  1. aType strain; other strain collection numbers: ATCC 43198, NCDO 2674. bBased on source of isolation and presence of clinical signs/pathology. cSmall colony variant strains of Enterococcus cecorum. dNormal colony morphology. e15 chicken eggs per isolate were inoculated with 102 CFU/egg into the allantoic cavity and examined for embryonic death for 7 days post inoculation. fTwo different antisera were produced from field strains D07-797-90/61 (Pekin duck, serotype 1) and 15/827/1/A (broiler, serotype 2) and isolates were tested using the slide agglutination procedure. gNon-typeable with the two produced antisera