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Table 1 Dead cell populations in control cultures and co-cultures of L. monocytogenes (A) and S. Enteritidis (B) by FCM analysis

From: Diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on Listeria monocytogenes and Salmonella enterica serovar enteritidis in a co-culture model

Bacteria

Day

Culture

Mean % dead cells

95% CI of mean

P value

A.

 

0

C

0.48

0.23–0.73

 

0

E

0.95

0.33–1.57

P > 0.10

8

C

3.03

1.20–4.86

 

8

E

12.63a

5.55–19.71

P < 0.05

15

C

4.13

−1.22–9.48

 

15

E

28.2a

15.18–41.21

P < 0.05

22

C

1.70

1.59–1.81

 

22

E

9.73a

5.44–14.02

P < 0.05

B.

 

0

C

0.68

0.02–1.34

 

0

E

1.80

1.62–1.98

P > 0.10

8

C

14.0

10.11–17.89

 

8

E

93.27b

88.0–98.53

P < 0.005

15

C

33.4

31.08–35.72

 

15

E

90.0b

87.95–92.05

P < 0.005

22

C

63.47

60.07–66.87

 

22

E

91.0b

87.75–94.25

P < 0.005

  1. Assessed using the t test. aandb indicate significantly higher dead cell population in co-cultures versus the corresponding control cultures of L. monocytogenes and S. Enteritidis respectively at the same day. Day 0 refers to time about 1 h after culture set-up. Mean is the percentage of total dead cells among three replicate assay tubes that are PI-stained. CI = confidence interval, C = control cultures, E = co-cultures