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Fig. 2 | BMC Microbiology

Fig. 2

From: Diffusible substances from lactic acid bacterial cultures exert strong inhibitory effects on Listeria monocytogenes and Salmonella enterica serovar enteritidis in a co-culture model

Fig. 2

Gating strategy to identify and quantitate total and dead bacteria. Cultured L.monocytogenes or S. Enteritidis were either untreated (Panels b, c) or heat-killed (d, e), diluted in PBS, and counting beads and propidium iodide (Panels b through e) added prior to acquisition. Bacteria were defined as events present within gate P1, and were distinct from events in the PBS buffer + beads control (Panel a). Counting beads were utilized as an internal control to normalize acquisition volume between samples, and were identified in gate P2. Dead cells were defined in gate P3 as propidium iodide-fluorescent positive events among P1-gated cells

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BMC Microbiology

ISSN: 1471-2180

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