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Table 2 Overview of Sf3 phage, ORFs, summary of homology searches and annotations
| ORF no. | Product | Strand | Begin | End | AA | Motif | Predicted functions | Homology score | E-value |
|---|---|---|---|---|---|---|---|---|---|
| ORF 1 | gp1 | + | 16 | 426 | 137 | - | - | - | - |
| ORF 2 | gp2 | + | 441 | 2099 | 553 | - | - | - | - |
| ORF 3 | gp3 | + | 2124 | 3551 | 476 | pfam05133 | Phage portal protein_Gp6 | 129 | 4.91e-33 |
| ORF 4 | gp4 | + | 3544 | 4344 | 267 | - | - | - | - |
| ORF 5 | gp5 | + | 4407 | 5156 | 250 | - | - | - | - |
| ORF 6 | gp6 | + | 5176 | 5565 | 130 | pfam02924 | Bacteriophage lambda head decoration protein D | 46,5 | 5.02e-08 |
| ORF 7 | gp7 | + | 5580 | 6626 | 349 | pfam03864 | Phage major capsid protein E | 62,35 | 3.13e-11 |
| ORF 8 | gp8 | + | 6623 | 6946 | 108 | - | - | - | - |
| ORF 9 | gp9 | + | 6952 | 7395 | 148 | pfam09355 | Phage protein Gp19 | 33,61 | 5.91e-03 |
| ORF 10 | gp10 | + | 7392 | 7745 | 118 | - | - | - | - |
| ORF 11 | gp11 | + | 7748 | 8026 | 93 | - | - | - | - |
| ORF 12 | gp12 | + | 8026 | 8427 | 134 | - | - | - | - |
| ORF 13 | gp13 | + | 8500 | 9165 | 222 | - | - | - | - |
| ORF 14 | gp14 | + | 9269 | 9592 | 108 | - | - | - | - |
| ORF 15 | gp15 | + | 9637 | 10065 | 143 | - | - | - | - |
| ORF 16 | gp16 | + | 10072 | 13644 | 1191 | pfam03864 | Phage major capsid protein E | 62,35 | 3.13e-11 |
| ORF 17 | gp17 | + | 13649 | 14539 | 297 | - | - | - | - |
| ORF 18 | gp18 | + | 14539 | 15684 | 382 | - | - | - | - |
| ORF 19 | gp19 | + | 15686 | 16612 | 309 | - | - | - | - |
| ORF 20 | gp20 | + | 16626 | 17243 | 206 | - | - | - | - |
| ORF 21 | gp21 | + | 17253 | 19277 | 675 | pfam12708 | Pectate lyase superfamily protein | 73,24 | 8.54e-15 |
| ORF 22 | gp22 | + | 19359 | 20186 | 276 | cd06583 | Peptidoglycan recognition proteins (PGRPs) | 58,45 | 4.03e-11 |
| ORF 23 | gp23 | + | 20233 | 20484 | 84 | - | - | - | - |
| ORF 24 | gp24 | + | 20528 | 20860 | 111 | COG4467 | YabA | 34,76 | 9.25e-04 |
| ORF 25 | gp25 | + | 20908 | 21546 | 213 | PHA03169 | hypothetical protein; Provisional | 35,72 | 5.62e-03 |
| ORF 26 | gp26 | + | 21757 | 22989 | 411 | cd00093 | Helix-turn-helix XRE-family like proteins. | 45,24 | 1.33e-06 |
| ORF 27 | gp27 | + | 23077 | 23346 | 90 | - | - | - | - |
| ORF 28 | gp28 | + | 23343 | 23537 | 65 | - | - | - | - |
| ORF 29 | gp29 | + | 23534 | 24052 | 173 | - | - | - | - |
| ORF 30 | gp30 | + | 24201 | 24683 | 161 | - | - | - | - |
| ORF 31 | gp31 | + | 24668 | 24781 | 38 | - | - | - | - |
| ORF 32 | gp32 | + | 24774 | 25223 | 150 | cd00075 | Histidine kinase-like ATPases | 36,09 | 4.50e-04 |
| ORF 33 | gp33 | - | 25247 | 25363 | 39 | - | - | - | - |
| ORF 34 | gp34 | + | 25319 | 25381 | 21 | - | - | - | - |
| ORF 35 | gp35 | + | 25382 | 26221 | 280 | pfam00730 | HhH-GPD superfamily base excision DNA repair protein | 47,36 | 3.71e-07 |
| ORF 36 | gp36 | + | 26181 | 27680 | 500 | - | - | - | - |
| ORF 37 | gp37 | + | 27677 | 28327 | 217 | cd01672 | Thymidine monophosphate kinase (TMPK) | 112 | 1.37e-30 |
| ORF 38 | gp38 | + | 28324 | 28755 | 144 | cd04683 | the Nudix hydrolase superfamily | 153 | 3.17e-48 |
| ORF 39 | gp39 | - | 29387 | 30592 | 402 | - | - | - | - |
| ORF 40 | gp40 | - | 30712 | 30963 | 84 | - | - | - | - |
| ORF 41 | gp41 | + | 30962 | 31105 | 48 | - | - | - | - |
| ORF 42 | gp42 | - | 31162 | 31524 | 121 | cd00093 | Helix-turn-helix XRE-family like proteins. | 41 | 1.99e-06 |
| ORF 43 | gp43 | + | 32113 | 32337 | 75 | - | - | - | - |
| ORF 44 | gp44 | + | 32425 | 32778 | 118 | - | - | - | - |
| ORF 45 | gp45 | + | 32771 | 33139 | 123 | - | - | - | - |
| ORF 46 | gp46 | + | 33136 | 33678 | 181 | - | - | - | - |
| ORF 47 | gp47 | + | 33675 | 33947 | 91 | - | - | - | - |
| ORF 48 | gp48 | + | 33944 | 34774 | 277 | pfam12705 | PD-(D/E)XK nuclease superfamily | 34,99 | 5.68e-03 |
| ORF 49 | gp49 | + | 34777 | 35838 | 354 | - | - | - | - |
| ORF 50 | gp50 | + | 35835 | 36647 | 271 | cd06127 | DEDDh 3’–5’ exonuclease domain family | 111 | 4.30e-30 |
| ORF 51 | gp51 | + | 36644 | 37099 | 152 | - | - | - | - |
| ORF 52 | gp52 | + | 37096 | 37713 | 206 | cd00529 | Holliday junction resolvases (HJRs) | 38,38 | 3.22e-04 |
| ORF 53 | gp53 | + | 37710 | 37985 | 92 | - | - | - | - |
| ORF 54 | gp54 | + | 37991 | 38428 | 146 | - | - | - | - |
| ORF 55 | gp55 | + | 38425 | 38775 | 117 | - | - | - | - |
| ORF 56 | gp56 | + | 38788 | 39564 | 259 | - | - | - | - |
| ORF 57 | gp57 | + | 39567 | 40202 | 212 | - | - | - | - |
| ORF 58 | gp58 | + | 40199 | 40402 | 68 | - | - | - | - |
| ORF 59 | gp59 | + | 40399 | 40926 | 176 | - | - | - | - |
| ORF 60 | gp60 | + | 40923 | 41120 | 66 | - | - | - | - |
| ORF 61 | gp61 | + | 41153 | 41506 | 118 | - | - | - | - |
| ORF 62 | gp62 | + | 41503 | 42369 | 289 | - | - | - | - |
| ORF 63 | gp63 | + | 42366 | 42692 | 109 | - | - | - | - |
| ORF 64 | gp64 | + | 42689 | 42814 | 42 | pfam10969 | Protein of unknown function (DUF2771) | 35,51 | 1.35e-04 |
| ORF 65 | gp65 | + | 42811 | 43368 | 186 | - | - | - | - |
| ORF 66 | gp66 | + | 43466 | 44308 | 281 | - | - | - | - |
| ORF 67 | gp67 | - | 44375 | 44590 | 72 | pfam02604 | Antitoxin Phd_YefM | 30,73 | 5.22e-03 |
| ORF 68 | gp68 | + | 44674 | 46254 | 527 | - | - | - | - |
| ORF 69 | gp69 | + | 46345 | 47619 | 425 | - | - | - | - |
| ORF 70 | gp70 | + | 47651 | 47743 | 31 | - | - | - | - |
| ORF 71 | gp71 | + | 47817 | 47996 | 60 | - | - | - | - |
| ORF 72 | gp72 | + | 48073 | 48510 | 146 | cd00397 | DNA breaking-rejoining enzymes | 40,54 | 2.27e-05 |
| ORF 73 | gp73 | - | 49011 | 49772 | 254 | - | - | - | - |
| ORF 74 | gp74 | + | 49506 | 49766 | 87 | - | - | - | - |
| ORF 75 | gp75 | + | 49841 | 49996 | 52 | - | - | - | - |
| ORF 76 | gp76 | + | 49993 | 50292 | 100 | cd00085 | HNH nucleases | 38,22 | 1.45e-05 |
| ORF 77 | gp77 | + | 50587 | 51171 | 195 | COG4983 | Uncharacterized protein | 79,98 | 9.19e-18 |
| ORF 78 | gp78 | + | 51278 | 51349 | 24 | - | - | - | - |
| ORF 79 | gp79 | - | 51714 | 52163 | 150 | - | - | - | - |
| ORF 80 | gp80 | - | 52167 | 53399 | 411 | - | - | - | - |
| ORF 81 | gp81 | - | 53709 | 54161 | 151 | - | - | - | - |
| ORF 82 | gp82 | + | 54647 | 55672 | 342 | pfam06381 | Protein of unknown function (DUF1073) | 39,22 | 1.07e-03 |
| ORF 83 | gp83 | - | 55695 | 55889 | 65 | - | - | - | - |
| ORF 84 | gp84 | + | 55948 | 56844 | 299 | TIGR01641 | phage putative head morphogenesis protein | 59,7 | 1.40e-11 |
| ORF 85 | gp85 | - | 56884 | 57198 | 105 | PRK13502 | transcriptional activator RhaR | 32,72 | 8.64e-03 |
| ORF 86 | gp86 | + | 57346 | 57642 | 99 | - | - | - | - |
| ORF 87 | gp87 | + | 57698 | 57847 | 50 | - | - | - | - |
| ORF 88 | gp88 | + | 58134 | 58682 | 183 | - | - | - | - |
| ORF 89 | gp89 | - | 58679 | 59956 | 426 | COG1783 | Phage terminase_3 | 161 | 6.32e-45 |
| ORF 90 | gp90 | - | 60233 | 60691 | 153 | - | - | - | - |
| ORF 91 | gp91 | - | 60684 | 60932 | 83 | - | - | - | - |
