From: Origins and global context of Brucella abortus in Italy
Branch | Location in B. abortus 2308 | SNP allele | Melt-MAMA primer | Melt-MAMA Primer sequences (5′ to 3′) | PCR mix Concentration | Annealing Temp. | Melting Temp. |
---|---|---|---|---|---|---|---|
Branch 1 West Italia | 951620 NC_007618.1 | C/T | A | GCGACCGCATCCTCTAaTC | 0.4 μM | 80.8 ° C | |
D | cggggcggggcggggGCGACCGCATCCTCTAcTT | 0.1 μM | 56 ° C | 83.4 ° C | |||
C | CATGGTAGGCAGGCTGGTT | 0.5 μM | |||||
Branch 2 | 1336380 | A/G | A | CGAAGTTTCCCGACACCcA | 0.4 μM | 81.2 ° C | |
West Italia | NC_007618.1 | D | cggggcggggcggggCGAAGTTTCCCGACACCtG | 0.1 μM | 56 ° C | 83.9 ° C | |
C | GATGAAGAAATCGATGAGCG | 0.5 μM | |||||
Branch 1 | 104052 | G/T | A | TGGAGGACGAAGTCTTCGaTG | 0.25 μM | 78 ° C | |
East Italia | NC_007618.1 | D | cggggcggggcggggGGAGGACGAAGTCTTCGcTT | 0.25 μM | 56 ° C | 82.9 ° C | |
C | ATCCTCGACAGTTGCGGC | 0.5 μM | |||||
Branch 2 | 360724 | C/A | A | TACCGCGGCCCGTCTAtC | 0.25 μM | 81.6 ° C | |
East Italia | NC_007618.1 | D | cggggcggggcggggTACCGCGGCCCGTCTAaA | 0.25 μM | 56 ° C | 83.7 ° C | |
C | AATCAAAGCGGCTTGCCTC | 0.5 μM | |||||
Branch 1 | 740090 | T/C | A | CAAAAGCGGACGGTCAgAT | 0.4 μM | 78.9 ° C | |
Trans Italia | NC_007618.1 | D | cggggcggggcggggTCAAAAGCGGACGGTCAtAC | 0.1 μM | 56 ° C | 80.9 ° C | |
C | CCCTCAACCCAGACGTTCG | 0.5 μM | |||||
Branch 2 | 864308 | C/T | A | AGGCATTACCGTTTCAGATTtC | 0.4 μM | 78.5 ° C | |
Trans Italia | NC_007618.1 | D | cggggcggggcggggAGGCATTACCGTTTCAGATTaT | 0.1 μM | 56 ° C | 81.9 ° C | |
C | TCTTTCCCGGCTCCGAAT | 0.5 μM |