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Table 3 Comparison of the 10 different algorithms tested on the 422 strains included in the assessment panel against the Bruker reference database

From: Decision criteria for MALDI-TOF MS-based identification of filamentous fungi using commercial and in-house reference databases

Threshold

A

B1

B2

C1

C2

C3

D1

D2

D3

D4

LS 1.5

%accepted

43.71

50.36

38.95

47.74

47.03

31.83

51.31

51.31

44.66

30.40

species PPV

0.46

0.47

0.43

0.51

0.51

0.46

0.50

0.50

0.48

0.42

genus PPV

0.65

0.67

0.57

0.70

0.70

0.61

0.69

0.69

0.66

0.54

LS 1.6

%accepted

35.15

40.62

29.22

40.38

39.67

25.18

44.42

44.42

38.00

24.23

species PPV

0.57

0.58

0.57

0.61

0.60

0.58

0.57

0.57

0.56

0.53

genus PPV

0.80

0.83

0.76

0.83

0.83

0.77

0.79

0.79

0.78

0.68

LS 1.7

%accepted

28.27

33.73

22.33

33.49

32.78

19.48

35.15

35.15

29.45

16.39

species PPV

0.71

0.70

0.74

0.73

0.72

0.76

0.72

0.72

0.73

0.78

genus PPV

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

LS 1.8

%accepted

17.58

23.75

19.48

24.47

23.99

17.58

25.89

25.89

22.33

15.20

species PPV

0.77

0.74

0.76

0.73

0.72

0.74

0.74

0.74

0.74

0.78

genus PPV

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

LS 1.9

%accepted

11.88

16.63

14.25

16.39

15.91

12.59

17.58

17.58

15.68

12.11

species PPV

0.78

0.77

0.77

0.77

0.76

0.75

0.77

0.77

0.74

0.78

genus PPV

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

LS 2.0

%accepted

7.84

10.21

9.74

10.45

10.21

9.03

10.45

10.45

9.98

8.79

species PPV

0.79

0.79

0.78

0.82

0.81

0.79

0.80

0.80

0.79

0.81

genus PPV

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

LS 2.1

%accepted

4.51

5.46

4.99

5.94

5.94

5.46

5.70

5.70

5.70

5.23

species PPV

0.79

0.78

0.76

0.80

0.80

0.78

0.75

0.75

0.75

0.77

genus PPV

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

LS 2.2

%accepted

3.33

4.04

3.56

3.56

3.56

3.09

3.80

3.80

3.80

3.56

species PPV

0.86

0.88

0.87

0.87

0.87

0.85

0.81

0.81

0.81

0.80

genus PPV

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

LS 2.3

%accepted

2.14

2.38

1.90

1.19

1.19

0.95

1.19

1.19

1.19

0.95

species PPV

0.78

0.80

0.75

1.00

1.00

1.00

1.00

1.00

1.00

1.00

genus PPV

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

1.00

  1. % accepted: percentage of submitted strains that fulfilled the criteria for identification at either the genus or species level, depending on the applied threshold; species PPV: positive predictive value at the species level; genus PPV: positive predictive value at the genus level