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Fig. 6 | BMC Microbiology

Fig. 6

From: Detailed analysis of c-di-GMP mediated regulation of csgD expression in Salmonella typhimurium

Fig. 6

Effect of c-di-GMP on csgD transcription in the S. typhimurium csgD mutant background. a Schematic drawing of fusion constructs containing the csgD promoter region of different length. Transcriptional activity of the csgD promoter region was analyzed in pUGE13 [25, 36] (b) upon deletion of STM1703 and STM4264 and (c) upon deletion of four diguanylate cyclases (Δ4DGCs) compared to the respective ΔcsgD::Km background The transcriptional activity was not affected in the Δ4DGC mutant whereas enhanced β-galactosidase activity was observed in Δ1703 compared to ΔcsgD:101. d-f Identification of the STM1703 regulatory region. As enhanced activity was observed only for pUGE5, but not for other fusions, STM1703 acts via the upstream region between nt −340 and −208. Controls were ΔryeB with partial reduction of csgD transcription [28] and ΔompR mutant as negative control. β-galactosidase measurements were done in duplicates using at least three technical replicates. Statistical significance is indicated by *P < 0.05 as compared to the respective ΔcsgD wild type control using unpaired t-test (Prism 5, GraphPad Software)

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