Fig. 5

Corresponding GGDEF/EAL domain proteins. Rdar morphotype and CsgD levels of S. typhimurium UMR1 upon deletion of the EAL domain protein STM3611 (a), the GGDEF-EAL protein STM1703 (b), and the EAL protein STM4264 (c) in different diguanylate cyclase mutant backgrounds. Alteration in rdar morphotype upon adrA deletion is due to lack of cellulose synthesis. Stereomicroscopic image of the rdar morphotype formation of the STM4264 mutant upon the deletion of four diguanylate cyclases after 48 h of growth at 28°C on LB without salts plates supplemented with Congo red (d) and without Congo red (e). Captions (a) 1 = UMR1Δ2123, 2 = UMR1Δ3611Δ2123, 3 = UMR1Δ3388, 4 = UMR1Δ3611Δ3388, 5 = UMR1Δ4551Δ1987, 6 = UMR1Δ3611Δ4551Δ1987. b 1 = UMR1Δ1703, 2 = UMR1Δ4551Δ1987Δ2123Δ3388, 3 = UMR1Δ1703Δ4551 Δ1987Δ2123Δ3388, 4 = Δ1703ΔAdrA 5 = Δ1703ΔAdrAΔ1283Δ2672. c 1 = UMRI, 2 = UMR1Δ4264, 3 = UMR1 Δ2123Δ3388, 4 = UMR1Δ4264Δ2123Δ3388, 5 = UMR1Δ4551Δ1987. 6 = UMR1Δ4264Δ4551Δ1987, 7 = UMR1Δ4551Δ1987Δ2123Δ3388, 8 = UMR1Δ4264Δ4551Δ1987Δ2123Δ3388