Fig. 2
From: Detailed analysis of c-di-GMP mediated regulation of csgD expression in Salmonella typhimurium
Cumulative effect of GGDEF proteins on rdar morphotype and csgD expression in S. typhimurium UMR1. a Rdar morphotype and CsgD levels of double and quadruple mutants of GGDEF proteins after 24 h of growth at 28°C on LB without salt agar plates. b Overexpression of the diguanylate cyclase STM4551 in the Δ4DGC mutant restored rdar morphotype and csgD expression, in contrast to catalytically inactive STM4551E267A. Cells were grown for 24 h at 28°C on LB without salt agar plates supplemented with ampicillin (100 μg ml−1) and 0.1% L-arabinose. VC = Vector control pBAD30, p4551 = STM4551 cloned in pBAD30, p4551E267A = catalytic mutant STM4551E267A cloned in pBAD30