Fig 7

ChMOB1 is required for septum formation and cell division. Two independent ΔChmob1 transformants (CY7242 and CY7243) and the parental strain ΔChku80 (CY6021) were tested for vegetative growth, virulence and the ability to produce septae. a Vegetative growth on oatmeal agar (OMA), potato dextrose agar (PDA) and Czapek-Dox minimal medium (MM). b A. thaliana 4 days after droplet inoculation. Representative images of trypan blue stained leaves are shown on the right. The quantification of appressoria (relative to total conidia titer used for infection), primary hyphae (relative to appressoria) and secondary hyphae (relative to primary hyphae) of this infection is shown on the left. The data of 3 plants per strain with 2 leaves per plant with at least 100 counted appressoria per leaf is given. Error bars are represented as the standard error from three replicates. Significant differences based on t-tests (p < 0.05) are marked with asterisks. c Axenic mycelium stained with calcofluor white. Septae are marked with white arrows. Scale bars = 20 μm