Fig. 4

ChMob2 co-localizes with ChCbk1 in the cytoplasm. Conidia of ΔChcbk1:: CBK1-mCherry ΔChmob2:: MOB2-GFP (CY6720) were rinsed off oatmeal plates and analyzed by CLSM during in vitro appressoria formation. a Conidia, b in vitro AP at 8 hpi, c in vitro AP at 18 hpi. Bright field, GFP and mCherry channels are shown from left to right. The rightmost picture shows an overlay of GFP and mCherry channels. The intensities between panels are not directly comparable, because different settings were used to optimally visualize protein localization. Scale bars = 20 μm