Protein or genetic element | Gene | Primers 5’ → 3’a |
Amplicon size (bpb) |
PCR conditions | References |
---|---|---|---|---|---|
Aae | aae |
F: GAGGAGGATTTTAAAGTGC R: AACATGACCATAGTAACC | 858 | 94 °C, 3 min; 40 cycles of: 94 °C, 90s; 55 °C, 1 min; 72 °C, 90s; final extension 72 °C, 5 min. | [8] |
Aap | aap |
F: ATACAACTGGTGCAGATGGTTG R: GTAGCCGTCCAAGTTTTACCAG | 400 | 94 °C, 3 min; 30 cycles of: 94 °C, 1 min; 50 °C, 1 min; 72 °C, 1 min; final extension 72 °C, 5 min. | [52] |
AtlE | atlE |
F: CAACTGCTCAACCGAGAACA R: TTTGTAGATGTTGTGCCCCA | 682 | 94 °C, 3 min; 30 cycles of: 94 °C, 1 min; 62 °C, 1 min; 72 °C, 1 min; final extension 72 °C, 5 min. | [33] |
Bhp | bhp |
F: ACGGACAATATCGTCTCTCAA R:: AACTTCAGCCGTTCCCTT | 1917 | 94 °C, 2 min; 40 cycles of: 94 °C, 30s; 55 °C, 30s; 72 °C, 75 s; final extension 72 °C, 5 min. | [10] |
Embp | embp |
F: AGCGGTACAAATGTCAATATC R: AGAAGTGCTCTAGCATCATCC | 455 | 94 °C, 3 min; 30 cycles of: 94 °C, 1 min; 62 °C, 1 min; 72 °C, 1 min; final extension 72 °C, 5 min. | [6] |
GehD | gehD |
F: TTTGAATTCTGCGCAAGCTCAATATAA R: TTTGCGGCCGCTATCGCTACTTACGTGTAA | 1179 | 94 °C, 2 min; 30 cycles of: 94 °C, 30s; 55 °C, 30s; 72 °C, 75 s; final extension 72 °C, 5 min. | [9] |
SdrF | sdrF |
F: GCTGAAGACAATCAATTAG R: TTAATATCCCCCTGTGCTG | 1875 | 94 °C, 4 min; 30 cycles of: 94 °C, 2 min; 60 °C, 1 min; 72 °C, 2 min; final extension 72 °C, 5 min. | [10] |
SdrG | fbe |
F: TAAACACCGACGATAATAACCAAA R: GGTCTAGCCTTATTTTCATATTCA | 496 | 94 °C, 3 min; 30 cycles of: 94 °C, 1 min; 62 °C, 1 min; 72 °C, 1 min; final extension 72 °C, 5 min. | [5] |
SesI | sesI |
F: GCTGATTATGTAAATGACTCAAAT R: AGCTTTTGTTGTTTGAGCTTC | 408 | 94 °C, 3 min; 30 cycles of: 94 °C, 1 min; 50 °C, 1 min; 72 °C, 1 min; final extension 72 °C, 5 min. | [11] |
IcaADB | icaADB |
F: TTATCAATG CCGCAGTTGTC R: GTTTAACGCGAGTGCGCTAT | 546 | 94 °C, 3 min; 30 cycles of: 94 °C, 1 min; 58 °C, 1 min; 72 °C, 1 min; final extension 72 °C, 5 min. | [33] |
ACME | arcA |
F: CTAACACTGAACCCCAATG R: GAGCCAGAAGTACGCGAG | 1946 | 95 °C, 10 min; 30 cycles of: 94 °C, 1 min; 52 °C, 1 min; 72 °C, 2 min; final extension 72 °C, 5 min. | [43] |
opp3AB |
F: GCAAATCTGTAAATGGTCTGTTC R: GAAGATTGGCAGCACAAAGTG | 1183 |