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Fig. 3 | BMC Microbiology

Fig. 3

From: A novel quantitative PCR detects Babesia infection in patients not identified by currently available non-nucleic acid amplification tests

Fig. 3

Detection of B. microti presence in the qPCR positive patient samples by IFA (Top Panels) Two representative samples (J22 and J67) from Ocean and Monmouth Counties not tested for Babesia by microscopy at JSUMC show green fluorescence due to reactivity of antibodies in patient plasma with the parasites followed by detection with Alexa 488 conjugated secondary antibodies, when observed by using FITC filter indicating positive IFA results (marked by arrows). (Bottom Panels) Blue fluorescence due to DAPI staining shows the parasites present (marked by arrows) in each field of view of the Nikon 80i fluorescence microscope at × 1000 magnification when Apo-Plan TIRF objective was used. Scales shown represent respective panels of Giemsa-stained microscopy and IFA

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BMC Microbiology

ISSN: 1471-2180

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