Interspecies transfer of vancomycin, erythromycin and tetracycline resistance among Enterococcus species recovered from agrarian sources

Table 5 Transferred antibiotic resistance genes identified by PCR

Transconjugant	Donor	Recipient	Selection conditions (μg/ml)	Genes transferred	Efficiency
T1	MF06036	MW1105^Rif	Vancomycin (10) + rifampicin (100)	vanA, ermB	7.8×10⁻³
T2	MF04010	MW1105^Rif	Tetracycline (10) + rifampicin (100)	Tet M Tet L	2.3X10⁻⁵
T3	MF06036	ST01109^Rif	Tetracycline (10) + rifampicin (100)	Tet M	1.8×10⁻⁴
T4	MF06035	MW1105^Rif	Vancomycin (10) + rifampicin (100)	vanA. ermB	1.22×10⁻¹

Solid plate mating reactions that resulted in the isolation of transconjugants. Donors and recipients were conjugated together on TSA and plated on selection media. Successful reactions were subjected to PCR and their transfer efficiency was calculated

ISSN: 1471-2180