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Fig. 3 | BMC Microbiology

Fig. 3

From: Optimisation of methods for bacterial skin microbiome investigation: primer selection and comparison of the 454 versus MiSeq platform

Fig. 3

Pictorial representation of expected versus the actual relative abundance observed for the individual components of the mock bacterial community on the MiSeq and Roche454 GS Junior platform for primer pair targeting the (a) V1-V3 hypervariable region and (b) V3-V4 hypervariable region. Note: In the case of Pseudomonadaceae the MiSeq was able to identify the bacterial component at very low abundance <1.1% relative abundance for both primer pairs but this was not detected in the Roche454 data. (For the observed relative abundances see Additional file 1: Table S1)

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BMC Microbiology

ISSN: 1471-2180

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