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Table 2 Comparison of the numbers of types generated by the various MLVA and PFGE. The numbers of types generated by the various MLVA using three (GECM-3), seven (GECM-7), or ten (GECM-10) loci, respectively, in comparison with that of PFGE using >80% similarity index for the designation of types, for the typing of 116 ESBL-E. coli isolates belonging to either the ST131-O25b linage (n = 34) or not

From: Evaluation of MLVA for epidemiological typing and outbreak detection of ESBL-producing Escherichia coli in Sweden

Number of isolates within a type GECM-3 GECM-7 GECM-10 PFGE
Non-O25b O25b Non-O25b O25b Non-O25b O25b Non-O25b O25b
Singleton 22 0 38 0 44 0 60a 6b
2–4 11 1 11 1 11 1 9b 7
5–9 2 0 3 0 2 0 0 0
≥10 1 2 0 2 0 2 0 1
Total number of types 36 3 52 3 57 3 69 14
Simpsons index of diversity (CI 95%) 0.96 (0.94–0.98) 0.59 - 0.98 (0.97–0.99) 0.59 - 0.99 (0.98–0.99) 0.59 - 1.0 (0.99–1.0) 0.89 -
  1. aIsolates non typeable by PFGE (n = 4) included, each with an individual designation and thus accounted for as singeltons. bPFGE type JB is included in both the non-O25b and O25b isolates (however both ST131), so it is listed in both columns
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BMC Microbiology

ISSN: 1471-2180

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