Fig. 3

In vitro response of the splenic lymphocytes of adult offspring rats to SEB. After the splenic lymphocytes of adult offspring rats were acquired in the PBS and SEB groups and in vitro co-cultured in RPMI medium with either SEB (named as PBS + SEB, SEB + SEB) or ConA (named as PBS + ConA, SEB + ConA) for 3 days, the percentages of CD4 and CD8 T cells in the splenocytes of adult female (a, b) and male (c, d) offspring rats were analyzed by flow cytometry. Values were calculated with data from 10 independent experiments [Twenty adult offspring rats (half male and half female) was used in each group.]. Data represent mean ± SE. Compared with PBS + ConA: & P < 0.05; Compared with SEB + ConA: * P < 0.05; Compared with PBS + SEB: # P < 0.05