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Table 2 Coverage and Shannon Index calculated using MOTHUR1 for each methanogen 16S rRNA gene clone library

From: Dietary pea fiber increases diversity of colonic methanogens of pigs with a shift from Methanobrevibacter to Methanomassiliicoccus-like genus and change in numbers of three hydrogenotrophs

Clone library OTUs observed CHAO1 OTU estimate % OTU coverage2 Shannon index ± 95% confidence limits Libshuff analysis
Piglet-C 10 10 99.49 1.47 ± 0.15a P < 0.0001
Piglet-P 11 11.5 98.95 1.53 ± 0.15b P < 0.0001
Finisher-C 10 10 99.48 1.66 ± 0.13c P < 0.0001
Finisher-P 15 15 99.49 2.08 ± 0.16d P < 0.0001
  1. 1Schloss et al
  2. 2Good’s coverage (C) according to the equation C = 1  (n/N), where n is the number of sequences represented by a single clone and N is the total
  3. number of clones in the library
  4. a,b,c,d There is significant difference between these values