Table 2 Plasmids and strains used in this study

 pETDuet-1

Amp^r oripBR322 lacI ^q T ₇ p

Novagen

 pACYCDuet-1

Cm^r oriP15A lacI ^q T ₇ p

Novagen

 pCP20

Cm^r, Amp^r pSC101 ori CI857

Novagen

 pA-accADBC

rep_p15A Cm^R lacI P_T7 accA P_T7 accD P_T7 accBC

[22]

 pET-phlDmar

rep_pBR322 kan^R lacI P_T7 phlD P_T7 mar

[22]

 pMCR-N-C

rep_pBR322 Amp^R lacI P_T7 His ₆ -mcr _1-549 P_T7 His ₆ -mcr _550-1219(N₉₄₀V/ K₁₁₀₆W/ S₁₁₁₄R)

[25]

  E. coli DH5α

F^- supE44 ΔlacU169 (ϕ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1

Invitrogen

  E. coli BL21(DE3)

F^- ompT gal dcm lon hsdSB (rB^- mB^-) λ(DE3)

Invitrogen

 JW3978

E. coli BW25113 iclR::kan

Keio collection

 Q1949

E. coli BL21(DE3)△arcA

[13]

 Q2133

E. coli BL21(DE3) iclR::kan

This study

 Q2280

E. coli BL21(DE3)△iclR

This study

 Q2135

E. coli BL21(DE3)△arcA iclR::kan

This study

 Q2138

E. coli BL21(DE3)△arcA△iclR

This study

 Q1944

E. coli BL21(DE3)/pA-accADBC/pET-phlDmar

This study

 Q1963

Q1949/pA-accADBC/pET-phlDmar

This study

 Q2283

Q2280/pA-accADBC/pET-phlDmar

This study

 Q2176

Q2138/pA-accADBC/pET-phlDmar

This study

 Q2204

E. coli BL21(DE3)/pA-accADBC/pMCR-N-C

This study

 Q2284

Q1949/pA-accADBC/pMCR-N-C

This study

 Q2285

Q2280/pA-accADBC/pMCR-N-C

This study

 Q2286

Q2138/pA-accADBC/pMCR-N-C

This study