Skip to main content

Table 2 Plasmids and strains used in this study

From: Improving the production of acetyl-CoA-derived chemicals in Escherichia coli BL21(DE3) through iclR and arcA deletion

Plasmids and strains Description Source
 pETDuet-1 Ampr oripBR322 lacI q T 7 p Novagen
 pACYCDuet-1 Cmr oriP15A lacI q T 7 p Novagen
 pCP20 Cmr, Ampr pSC101 ori CI857 Novagen
 pA-accADBC repp15A CmR lacI PT7 accA PT7 accD PT7 accBC [22]
 pET-phlDmar reppBR322 kanR lacI PT7 phlD PT7 mar [22]
 pMCR-N-C reppBR322 AmpR lacI PT7 His 6 -mcr 1-549 PT7 His 6 -mcr 550-1219(N940V/ K1106W/ S1114R) [25]
E. coli DH5α F- supE44 ΔlacU169 (ϕ80 lacZ ΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Invitrogen
E. coli BL21(DE3) F- ompT gal dcm lon hsdSB (rB- mB-) λ(DE3) Invitrogen
 JW3978 E. coli BW25113 iclR::kan Keio collection
 Q1949 E. coli BL21(DE3)arcA [13]
 Q2133 E. coli BL21(DE3) iclR::kan This study
 Q2280 E. coli BL21(DE3)iclR This study
 Q2135 E. coli BL21(DE3)arcA iclR::kan This study
 Q2138 E. coli BL21(DE3)arcAiclR This study
 Q1944 E. coli BL21(DE3)/pA-accADBC/pET-phlDmar This study
 Q1963 Q1949/pA-accADBC/pET-phlDmar This study
 Q2283 Q2280/pA-accADBC/pET-phlDmar This study
 Q2176 Q2138/pA-accADBC/pET-phlDmar This study
 Q2204 E. coli BL21(DE3)/pA-accADBC/pMCR-N-C This study
 Q2284 Q1949/pA-accADBC/pMCR-N-C This study
 Q2285 Q2280/pA-accADBC/pMCR-N-C This study
 Q2286 Q2138/pA-accADBC/pMCR-N-C This study