Fig. 4

Maximum likelihood phylogeny constructed using a concatenated alignment of conserved mitochondrial protein sequences. The concatenated amino acid alignment of the conserved protein coding genes Atp6, Atp8, Atp9, Cob, Cox1, Cox2 and Cox3 was used to construct a phylogenetic tree. The isolate C. subhashii FGA 2.2 is shown in bold and the CTG clade is marked in green. Yarrowia lipolytica was used as the outgroup. As the concatenated protein sequences encoded by the seven genes of the previously published and the Swiss C. subhashii strains were identical, the branch length on the respective node was zero (in red). Bootstrap scores for all nodes are shown (percentage of 100 bootstrap runs). The bar represents the number of amino acid substitutions per site. Further information about the selected sequences is reported in Table 2