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Fig. 4 | BMC Microbiology

Fig. 4

From: Acanthamoeba containing endosymbiotic chlamydia isolated from hospital environments and its potential role in inflammatory exacerbation

Fig. 4

IL-8 induction in Protochlamydia W-9-stimulated HEp-2 cell cultures. a Assessment of IL-8 gene expression in cultures (24 h post-infection). The level of IL-8 gene expression was determined according to qPCR using primer sets specific to IL-8 and an internal control (GAPDH: glyceraldehyde-3-phosphate dehydrogenase). The data represent the average copies ± SD. b Amounts of IL-8 in culture supernatants. The amounts of IL-8 were quantified with a commercial kit, Human IL-8 ELISA MAX™ Deluxe. The data represent the average amount ± SD (pg/ml). * indicates p < 0.05

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BMC Microbiology

ISSN: 1471-2180

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