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Fig. 3 | BMC Microbiology

Fig. 3

From: In vitro rescue of genital strains of Chlamydia trachomatis from interferon-γ and tryptophan depletion with indole-positive, but not indole-negative Prevotella spp.

Fig. 3

Recovery of tryptophan-starved C. trachomatis strain D after rescue with exogenous tryptophan (122–980 μM) or indole (0.1–10 μM). Monolayers of HEp-2 cells were seeded in the presence of tryptophan-depleted media 24 h before infection. Cells were infected with C. trachomatis D, at an MOI of 0.5, and were incubated for 36 h. The Chlamydia infected cultures were allowed to recover for 36 h with increasing concentrations of tryptophan (a) or indole (b). Infected cells and culture supernatants were sonicated and used to infect a new HEp-2 cell monolayer for enumeration of recoverable IFUs. Data are presented as the mean ± SD IFU/ml (n = 9) determinations

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