Fig. 2

Effect of different indole concentrations on the recovery of C. trachomatis D following IFN-γ treatment. Monolayers of HEp-2 cells were seeded 48 h before infection in the presence of different IFN-γ concentrations (0, 150, 500 U/ml). IFN-γ treatment was replenished every 24 h throughout the whole experiment. Cells were infected with C. trachomatis D, at an MOI of 0.5, and were incubated for 36 h. The Chlamydia infected cultures were allowed to recover for 24 h with different indole concentrations (0.1, 0.25, 0.5, 1 μM). Infected cells and culture supernatants were sonicated and used to infect a new HEp-2 cell monolayer for enumeration of recoverable IFUs. Data are presented as the mean ± SD IFU/ml (n = 9) determinations