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Table 1 The strains and plasmids used in this study

From: Disrupting ROS-protection mechanism allows hydrogen peroxide to accumulate and oxidize Sb(III) to Sb(V) in Pseudomonas stutzeri TS44

Strain/plasmid Relevant properties or derivation Source or reference
Pseudomonas stutzeri
TS44 Wild-type, As(III)-oxidizing phenotype [27]
TS44-gshA540 Kanr; gshA mutant by Tn5 random transposon mutagenesis This study
TS44-gshA-C Kanr Cmr; gshA complemented strain This study
TS44 (PgshA) Kanr; TS44 with pLSP-PgshA This study
TS44 (PsodB) Kanr; TS44 with pLSP-PsodB This study
TS44 (PsodC) Kanr; TS44 with pLSP-PsodC This study
TS44-gshA540
(PgshA)
Kanr; TS44-gshA540 with pLSP-PgshA This study
TS44-gshA540
(PsodB)
Kanr; TS44-gshA540 with pLSP-PsodB This study
TS44-gshA540
(PsodC)
Kanr; TS44-gshA540 with pLSP-PsodC This study
Escherichia coli
DH5α supE44 lacU169(j80lacZM15) hRDR17 recA1
endA1 gyrA96 thi-1 relA1
[35]
S17-1 Pro Mob+; conjugation donor [36]
pir116 mcrA, Δ(mrr-hsdRMS-mcrBC) recA1 R6Kγ lacZΔM15 λpir Epicenter, Madison, WI
Plasmids
pRL27-Kan Kanr Transposon vector, oriR6K [37]
pCT-Zori broad host vector, pUC ori, Cmr [34]
pCT-Zori-gshA gshA complementation vector This study
pLSP-kt2lacZ Kanr oriV; lacZ fusion vector used for lacZ fusion constructs T. R. McDermott, MSU
pLSP-PgshA pLSP-kt2lacZ containing gshA promoter region This study
pLSP-PsodB pLSP-kt2lacZ containing sodB promoter region This study
pLSP-PsodC pLSP-kt2lacZ containing sodC promoter region This study