Fig. 6
miR-3473 expressed significantly in lung cells from Bp-infected mice, involved in the TNF-α release and animal survival. a TNF-α release was measured by ELISA on blood samples from Bp, Bt-infected or uninfected mice on the fourth day after infection (4 dpi, with the dose of 2 × 105 CFU Bp or 8 × 107 CFU Bt or no bacteria, both in 50 μL PBS and injected into mice). b Lung cells were separated from Bp-infected, Bt-infected or uninfected mice at 4 dpi. and miR-3473 expression was measured by qRT-PCR described as above. c miR-3473 inhibitor oligonucleotide was administrated to mice (i.n., through breathing) on the day before inoculation and TNF-α release was measured at 4 dpi. d Survival curves of mice infected with Bp, Bt or PBS for 7 days with or without treatment of miR-3473 inhibitor oligonucleotides. *P value < 0.05, **P value < 0.01