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Fig. 5 | BMC Microbiology

Fig. 5

From: Burkholderia pseudomallei-derived miR-3473 enhances NF-κB via targeting TRAF3 and is associated with different inflammatory responses compared to Burkholderia thailandensis in murine macrophages

Fig. 5

miR-3473 regulated TNF-α release specifically, but did not influence bacterial replication in Bp-treated macrophages. a qRT-PCR analysis of TNF-α mRNA expression in Bp-infected or uninfected macrophages pre-treated with miR-3473 mimic, inhibitor or controls. b TNF-α mRNA levels were measured in Bp, Bt-infected or uninfected macrophages pre-treated with microRNA oligonucleotides. c Early apoptosis cells were gated through flow cytometry test in macrophages pre-transfected with miR-3473 inhibitor or control at 12 h after Bp or Bt infection. d Intracellular bacteria loads of Bp or Bt-infected macrophages transfected with miR-3473 oligonucleotides were counted at different infectious phase (4 h, 8 h, 12 h, 20 h and 28 h). *P value < 0.05, **P value < 0.01

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BMC Microbiology

ISSN: 1471-2180

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