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Probe_norf1_fw
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GTAATACGACTCACTATAGGGAGACCATCGACTATTCTTCAGTACTGTTTAC
|
Amplification of probe template norf1 (168 nt) for in vitro-transcription (T7 promoter is underlined)
|
|
Probe_norf1_rev
|
TTGAGATGCTACAGGACCTTATGC
|
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Pnorf1_fw
|
taccggtGCCTAGGGGATACCTCTCCCC
|
Amplification of putative norf1 promoter for ligation into pILA reporter plasmid
|
|
Pnorf1_rev
|
tggccggcCTCCGTCCCAATGGGGGAAAC
|
|
nsiR6_probe_fw
|
GTAATACGACTCACTATAGGGAGATTACCGATCGCCGCTTCATC
|
Amplification of probe template nsiR6 (166 nt) for in vitro-transcription (T7 promoter is underlined)
|
|
nsiR6_probe_rev
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TGTGTGGCGTCACCATTGAAAATG
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hliR1_probe_fw
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GTAATACGACTCACTATAGGGAGACTCGGGAAGATTAAGACTGGTTTTG
|
Amplification of probe template hliR1 (135 nt) for in vitro-transcription (T7 promoter is underlined)
|
|
hliR1_probe_rev
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ATGTCTAATTTGATTGCTGTTGCTTTC
|
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norf4_probe_fw
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GTAATACGACTCACTATAGGGAGACCCCCTTTAGCAAAACTACCCATC
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Amplification of probe template norf4 (116 nt) for in vitro-transcription (T7 promoter is underlined)
|
|
norf4_probe_rev
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ATGACCGCCGATCAACTGTTG
|
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nsiR6_fw
|
gccaagaagtATGAGTGTTTTCCCCGCAGA
|
Amplification of nsiR6 generating overlaps with PpetE and 3xFLAG::3′UTR norf1::Toop
|
|
nsiR6_rev
|
tataatccatGTCGTAATAATCCCGGCTGG
|
|
PpetE::hliR1_fw
|
gccaagaagtATGTCTAATTTGATTGCTGTTG
|
Amplification of hliR1 generating overlaps with PpetE and 3xFLAG::3′UTR norf1::Toop
|
|
3xFlag_hliR1_rev
|
tataatccatCTCGGGAAGATTAAGACTGG
|
|
PpetE::ssr1169_fw
|
gccaagaagtATGGATATTGTTAAGATCATTTGTGCGATTC
|
Amplification of ssr1169 generating overlaps with PpetE and 3xFLAG::3′UTR norf1::Toop
|
|
3xFlag_ssr1169_rev
|
tataatccatACGTTCCCTGGCAATGACCC
|
|
PpetE::Norf4_fw
|
gccaagaagtATGACCGCCGATCAACTGTT
|
Amplification of n
orf4 generating overlaps with PpetE and 3xFLAG::3′UTR n
orf1::Toop
|
|
3xFlag_Norf4_rev
|
tataatccatACCCCCTTTAGCAAAACTAC
|
|
pUC19-XbaI_PpetE_fw
|
gctcggtacccggggatcctctagaCTGGGCCTACTGGGCTATTC
|
Amplification of PpetE introducing XbaI site (underlined) + creating overlaps with pUC19 and nsiR1, hliR
1, ssr1169 or n
orf4
|
|
nsiR6::PpetE_rev
|
aaacactcatACTTCTTGGCGATTGTATCTATAGG
|
|
hliR1::PpetE_rev
|
aattagacatACTTCTTGGCGATTGTATCTATAGG
|
|
ssr1169::PpetE_rev
|
caatatccatACTTCTTGGCGATTGTATCTATAGG
|
|
Norf4::PpetE_rev
|
gatcggcggtcatACTTCTTGGCGATTGTATCTATAGG
|
|
3xFlag_PstI-pUC19_rev
|
gccaagcttgcatgcctgcagAATAAAAAACGCCCGGCGGC
|
Amplification of 3xFLAG::3′UTR norf1::Toop introducing PstI site (underlined) + creating overlaps with pUC19 and nsiR1, hlirR, ssr1169 or norf4 or amplification of particular CDS associated with 3xFLAG__3′UTR norf1::Toop introducing PstI site (underlined) + creating overlaps with pUC19 and respective promoter sequence
|
|
nsiR6_3xFlag_fw
|
ttattacgacATGGATTATAAAGATCATGATGGCGATTATAAAG
|
|
hliR1_3xFlag_fw
|
tcttcccgagATGGATTATAAAGATCATGATGGCGATTATAAAG
|
|
ssr1169_3xFlag_fw
|
cagggaacgtATGGATTATAAAGATCATGATGGCGATTATAAAG
|
|
Norf4_3xFlag_fw
|
taaagggggtATGGATTATAAAGATCATGATGGCGATTATAAAG
|
|
CDSnsiR6::PnsiR6_fw
|
ataaatactcATGAGTGTTTTCCCCGCAGAAAC
|
|
CDShliR1::PhliR1_fw
|
aaattaactaaATGTCTAATTTGATTGCTGTTGCTTTCTG
|
|
CDSNorf4::PNorf4_fw
|
aatttttaccATGACCGCCGATCAACTGTTG
|
|
CDSssr1169::Pssr1169_fw
|
gagtgaactaATGGATATTGTTAAGATCATTTGTGCGATTC
|
|
pUC19::PnsiR6_fw
|
gctcggtacccggggatcctctagaATCGCCGTATTACACCTCTG
|
Amplification of PnsiR6 introducing XbaI site (underlined) + generating overlaps with pUC19 and nsiR6.
|
|
PnsiR6::CDSnsiR6_rev
|
aaacactcatGAGTATTTATTCCTAGTGAATGAATTAGAAG
|
|
pUC19::PhliR1_fw
|
gctcggtacccggggatcctctagaGGAGTTTACAGCGAGATTTG
|
Amplification of PhliR1 introducing XbaI site (underlined) + generating overlaps with pUC19 and hliR1.
|
|
PhliR::CDShliR1_rev
|
aattagacatTTAGTTAATTTTTGTAACGGGAG
|
|
pUC19::PNorf4_fw
|
gctcggtacccggggatcctctagaAGGTGATGATTATGAGCCGTC
|
Amplification of Pnorf4 introducing XbaI site (underlined) + generating overlaps with pUC19 and norf4.
|
|
PNorf4::CDSNorf4_rev
|
gatcggcggtcatGGTAAAAATTCCACTAATTCAAAAAAC
|
|
pUC19::Pssr1169_fw
|
gctcggtacccggggatcctctagaCGAGTAGCCAGCCAAAGCAG
|
Amplification of Pssr1169 introducing XbaI site (underlined) + generating overlaps with pUC19 and ssr1169.
|
|
Pssr1169::CDSssr1169_rev
|
caatatccatTAGTTCACTCCAATATGTCGGGATAATTAG
|
