Probe_norf1_fw
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GTAATACGACTCACTATAGGGAGACCATCGACTATTCTTCAGTACTGTTTAC
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Amplification of probe template norf1 (168 nt) for in vitro-transcription (T7 promoter is underlined)
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Probe_norf1_rev
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TTGAGATGCTACAGGACCTTATGC
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Pnorf1_fw
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taccggtGCCTAGGGGATACCTCTCCCC
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Amplification of putative norf1 promoter for ligation into pILA reporter plasmid
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Pnorf1_rev
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tggccggcCTCCGTCCCAATGGGGGAAAC
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nsiR6_probe_fw
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GTAATACGACTCACTATAGGGAGATTACCGATCGCCGCTTCATC
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Amplification of probe template nsiR6 (166 nt) for in vitro-transcription (T7 promoter is underlined)
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nsiR6_probe_rev
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TGTGTGGCGTCACCATTGAAAATG
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hliR1_probe_fw
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GTAATACGACTCACTATAGGGAGACTCGGGAAGATTAAGACTGGTTTTG
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Amplification of probe template hliR1 (135 nt) for in vitro-transcription (T7 promoter is underlined)
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hliR1_probe_rev
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ATGTCTAATTTGATTGCTGTTGCTTTC
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norf4_probe_fw
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GTAATACGACTCACTATAGGGAGACCCCCTTTAGCAAAACTACCCATC
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Amplification of probe template norf4 (116 nt) for in vitro-transcription (T7 promoter is underlined)
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norf4_probe_rev
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ATGACCGCCGATCAACTGTTG
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nsiR6_fw
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gccaagaagtATGAGTGTTTTCCCCGCAGA
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Amplification of nsiR6 generating overlaps with PpetE and 3xFLAG::3′UTR norf1::Toop
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nsiR6_rev
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tataatccatGTCGTAATAATCCCGGCTGG
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PpetE::hliR1_fw
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gccaagaagtATGTCTAATTTGATTGCTGTTG
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Amplification of hliR1 generating overlaps with PpetE and 3xFLAG::3′UTR norf1::Toop
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3xFlag_hliR1_rev
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tataatccatCTCGGGAAGATTAAGACTGG
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PpetE::ssr1169_fw
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gccaagaagtATGGATATTGTTAAGATCATTTGTGCGATTC
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Amplification of ssr1169 generating overlaps with PpetE and 3xFLAG::3′UTR norf1::Toop
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3xFlag_ssr1169_rev
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tataatccatACGTTCCCTGGCAATGACCC
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PpetE::Norf4_fw
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gccaagaagtATGACCGCCGATCAACTGTT
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Amplification of n
orf4 generating overlaps with PpetE and 3xFLAG::3′UTR n
orf1::Toop
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3xFlag_Norf4_rev
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tataatccatACCCCCTTTAGCAAAACTAC
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pUC19-XbaI_PpetE_fw
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gctcggtacccggggatcctctagaCTGGGCCTACTGGGCTATTC
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Amplification of PpetE introducing XbaI site (underlined) + creating overlaps with pUC19 and nsiR1, hliR
1, ssr1169 or n
orf4
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nsiR6::PpetE_rev
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aaacactcatACTTCTTGGCGATTGTATCTATAGG
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hliR1::PpetE_rev
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aattagacatACTTCTTGGCGATTGTATCTATAGG
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ssr1169::PpetE_rev
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caatatccatACTTCTTGGCGATTGTATCTATAGG
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Norf4::PpetE_rev
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gatcggcggtcatACTTCTTGGCGATTGTATCTATAGG
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3xFlag_PstI-pUC19_rev
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gccaagcttgcatgcctgcagAATAAAAAACGCCCGGCGGC
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Amplification of 3xFLAG::3′UTR norf1::Toop introducing PstI site (underlined) + creating overlaps with pUC19 and nsiR1, hlirR, ssr1169 or norf4 or amplification of particular CDS associated with 3xFLAG__3′UTR norf1::Toop introducing PstI site (underlined) + creating overlaps with pUC19 and respective promoter sequence
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nsiR6_3xFlag_fw
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ttattacgacATGGATTATAAAGATCATGATGGCGATTATAAAG
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hliR1_3xFlag_fw
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tcttcccgagATGGATTATAAAGATCATGATGGCGATTATAAAG
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ssr1169_3xFlag_fw
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cagggaacgtATGGATTATAAAGATCATGATGGCGATTATAAAG
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Norf4_3xFlag_fw
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taaagggggtATGGATTATAAAGATCATGATGGCGATTATAAAG
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CDSnsiR6::PnsiR6_fw
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ataaatactcATGAGTGTTTTCCCCGCAGAAAC
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CDShliR1::PhliR1_fw
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aaattaactaaATGTCTAATTTGATTGCTGTTGCTTTCTG
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CDSNorf4::PNorf4_fw
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aatttttaccATGACCGCCGATCAACTGTTG
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CDSssr1169::Pssr1169_fw
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gagtgaactaATGGATATTGTTAAGATCATTTGTGCGATTC
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pUC19::PnsiR6_fw
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gctcggtacccggggatcctctagaATCGCCGTATTACACCTCTG
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Amplification of PnsiR6 introducing XbaI site (underlined) + generating overlaps with pUC19 and nsiR6.
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PnsiR6::CDSnsiR6_rev
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aaacactcatGAGTATTTATTCCTAGTGAATGAATTAGAAG
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pUC19::PhliR1_fw
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gctcggtacccggggatcctctagaGGAGTTTACAGCGAGATTTG
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Amplification of PhliR1 introducing XbaI site (underlined) + generating overlaps with pUC19 and hliR1.
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PhliR::CDShliR1_rev
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aattagacatTTAGTTAATTTTTGTAACGGGAG
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pUC19::PNorf4_fw
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gctcggtacccggggatcctctagaAGGTGATGATTATGAGCCGTC
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Amplification of Pnorf4 introducing XbaI site (underlined) + generating overlaps with pUC19 and norf4.
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PNorf4::CDSNorf4_rev
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gatcggcggtcatGGTAAAAATTCCACTAATTCAAAAAAC
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pUC19::Pssr1169_fw
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gctcggtacccggggatcctctagaCGAGTAGCCAGCCAAAGCAG
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Amplification of Pssr1169 introducing XbaI site (underlined) + generating overlaps with pUC19 and ssr1169.
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Pssr1169::CDSssr1169_rev
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caatatccatTAGTTCACTCCAATATGTCGGGATAATTAG
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