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Table 3 Total sterol and carotenoid contents in X. dendrorhous thiolase overexpression strains

From: Functional characterization of thiolase-encoding genes from Xanthophyllomyces dendrorhous and their effects on carotenoid synthesis

Strain

UCD 67–385

385-Vexp2

385-POT1

385-ERG10

Gene overexpression (−fold compared to control)

N/A

1.0 (0.9–1.1)

4.9*(3.7–6.4)

3.2*(2.2–4.7)

Total sterols (mg/g dry weight)

3.5 ± 0.4

3.8 ± 0.1

3.9 ± 0.2

3.9 ± 0.1

Total carotenoids (μg/g dry weight)

359.0 ± 16.3

401.7 ± 26.8

394.1 ± 15.9

349.7 ± 24.8

% Astaxanthin

75.4 ± 1.2

74.4 ± 0.2

68.7 ± 2.0

73.8 ± 1.4

% Beta-carotene

2.8 ± 0.7

2.6 ± 0.1

4.1 ± 0.9

2.8 ± 0.4

% Phoenicoxanthin

11.6 ± 0.3

10.8 ± 0.7

12.2 ± 0.6

10.9 ± 0.6

  1. Each value corresponds to the average value of three independent samples ± standard deviation. Overexpression data are presented as an average with standard deviation in parentheses. The relative expression of the studied genes in 385-Vexp2 was compared to wild type strain UCD 67–385, and the results indicated no significant differences in the relative expression of these genes; therefore, relative expression for each gene in the overexpression mutants was compared to control strain 385-Vexp2. N/A = Not Analyzed. *p < 0.01, Student’s T-test