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Table 2 Total sterol and carotenoid content in X. dendrorhous POT1 mutant strains

From: Functional characterization of thiolase-encoding genes from Xanthophyllomyces dendrorhous and their effects on carotenoid synthesis

Strain

UCD 67–385

385-pot1(+/−)

385-pot1(−/−)

Total Sterols (mg/g dry weight)

5.7 ± 0.3

4.6 ± 0.2

5.6 ± 0.6

Total Carotenoids (μg/g dry weight)

207.8 ± 21.5

201.5 ± 3.8

174 ± 19.5

% Astaxanthin

82.0 ± 3.4

78.6 ± 0.7

49.4* ± 2.4

% Beta-carotene

2.3 ± 0.7

2.8 ± 0.1

10.0* ± 0.8

% Phoenicoxanthin

8.0 ± 1.3

9.9 ± 0.4

16.5* ± 0.6

% Other carotenoids

7.7 ± 1.7

8.6 ± 0.3

23.0* ± 2.0

  1. Each value corresponds to the average of three independent samples ± standard deviation. Other carotenoids include hydroxy-keto-γ-carotene, hydroxy-equinenone, hydroxy-keto-torulene and canthaxanthin. *p < 0.01, Student’s T-test compared to wild type