Fig. 4

Changes in transcript levels for POT1 mutant strains. Relative transcript levels of genes POT1, ERG10, crtS and crtR of X. dendrorhous were analyzed in samples obtained after 96 h of growth in liquid YM media for strains UCD 67–385, 385-pot1 ^(+/−) and 385-pot1 ^(−/−). The results were analyzed using the 2^-ΔΔCt method using actin as the normalizer gene and UCD 67–385 as the control strain compared to 385-pot1 ^(+/−) (blue) and 385-pot1 ^(−/−) (red). For POT1 transcript analysis in strain 385-pot1 ^(−/−), no transcript was detected. Each bar represents an average of three independent samples. Black lines indicate standard deviation. *p < 0.01