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Table 4 Vaginal microbiome species profiling

From: Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification

  Singleton-free unique OTU and no. of species
MiSeq V1V2 129 OTUs = 70 species
Subsampled to 27596
PacBio in silico V1V2 154 OTUs = 63 species
Minscore = 80 Minsim = 80 (39 species (58%) shared with MiSeq V1V2)
PacBio in silico V1V2 131 OTUs = 48 species
Minscore = 90 Minsim = 90 (26 species (37%) shared with MiSeq V1V2)
PacBio V1V9 261 OTUs = 52 species
Minscore = 80 minsim = 80 (28 species (40%) shared with MiSeq V1V2)
(32 species (62%) shared with PacBio in silico V1V2, minscore = 80, minsim = 80)
PacBio V1V9 148 OTUs = 48 species
Minscore = 90 minsim = 90 (26 species (37%) shared with MiSeq V1V2)
(48 species (100%) shared with PacBio in silico V1V2, minscore = 90, minsim = 90)
  1. Abbreviations: minscore minimum alignment score during post alignment screening, minsim minimum similarity score during post alignment screening, OTU operational taxonomic units, MiSeq Illumina MiSeq sequencing, V1V2 bacterial 16S rRNA V1V2 gene region, V1V9 full-length bacterial 16S rRNA gene